| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PARP1 recombinant protein (Position: D6-R841) was used as the immunogen for the PARP1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PARP1 Antibody / Poly ADP-ribose polymerase 1 is a anti-PARP1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: PARP1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, FACS, ELISA
Biological background
Functionally, PARP1 antibody identifies a 1014-amino-acid nuclear protein containing three zinc-finger DNA-binding domains, a BRCT motif, and a catalytic PARP domain. Upon binding to damaged DNA, PARP1 catalyzes the transfer of ADP-ribose units from NAD+ to target proteins, forming long poly-ADP-ribose (PAR) chains. These modifications facilitate the recruitment of DNA repair factors such as XRCC1, DNA ligase III, and DNA polymerase beta. PARP1 activity is a key step in the base excision repair (BER) pathway, ensuring timely resolution of single-strand breaks.
The PARP1 gene is located on chromosome 1q42.12 and is ubiquitously expressed in proliferative and differentiated tissues. It modulates chromatin relaxation by poly-ADP-ribosylating histones, thereby granting access to repair and transcriptional complexes. Beyond DNA repair, PARP1 regulates transcription by interacting with nuclear receptors and chromatin modifiers, influencing inflammation, cell cycle, and apoptosis. PARP1 activity is tightly regulated by DNA damage signals and cellular NAD+ levels.
In apoptosis, excessive PARP1 activation during oxidative or genotoxic stress leads to NAD+ and ATP depletion, contributing to programmed necrosis (parthanatos). PARP inhibitors, such as olaparib and niraparib, target PARP1 enzymatic activity to enhance the cytotoxicity of DNA-damaging agents, forming the basis for synthetic lethality in BRCA-deficient cancers. Overactivation or dysregulation of PARP1 has been linked to neurodegeneration, ischemic injury, and cancer progression.
PARP1 antibody is widely used in molecular biology, oncology, and DNA repair research. It is suitable for western blotting, immunohistochemistry, and chromatin immunoprecipitation to detect endogenous or modified PARP1. This antibody supports studies of DNA repair, PARP inhibitor mechanisms, and nuclear signaling pathways. In cancer models, PARP1 detection is crucial for assessing DNA damage response and drug sensitivity.
Structurally, PARP1 contains DNA-binding zinc fingers, a WGR domain involved in DNA interaction, and a catalytic domain responsible for NAD+ hydrolysis and ADP-ribose polymerization. Its activity is regulated through automodification, phosphorylation, and proteolytic cleavage during apoptosis.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
