| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human PARP11 was used as the immunogen for the PARP11 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PARP11 Antibody / Poly ADP-ribose polymerase 11 is a anti-PARP11 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: PARP11
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, ICC/IF, FACS
Biological background
PARP11 is encoded by the PARP11 gene on human chromosome 12q13.11. The protein is approximately 39 kilodaltons and characterized by an N-terminal WWE domain, which mediates protein-protein interactions, and a C-terminal catalytic domain containing the conserved histidine-tyrosine-glutamate motif typical of ADP-ribosyltransferases. PARP11 localizes predominantly to the nuclear envelope and cytoplasmic membrane-associated compartments.
The PARP11 antibody detects a 39 kilodalton band by western blot and shows nuclear rim and cytoplasmic punctate staining patterns in immunofluorescence microscopy. PARP11 functions in antiviral defense by ADP-ribosylating host and viral proteins involved in interferon signaling, modulating downstream immune activation. It targets nuclear pore complex components and transport receptors, thereby altering nucleocytoplasmic trafficking during stress or infection. Studies have demonstrated that PARP11 negatively regulates type I interferon responses by promoting degradation of TBK1 and IFNAR1, helping fine-tune innate immune activity.
PARP11 also influences ubiquitin-proteasome and SUMOylation pathways through crosstalk with other post-translational modifiers. It plays a role in maintaining nuclear envelope structure and in mitotic progression. Overexpression of PARP11 has been associated with enhanced cell survival under stress, whereas its inhibition sensitizes cells to DNA damage and viral infection. Structural studies suggest that PARP11 recognizes unique NAD+ analogs distinct from other PARP family members, indicating potential for selective drug targeting.
Emerging evidence links PARP11 to cancer, viral replication, and neurodegenerative processes. In hepatocellular and colorectal carcinomas, PARP11 overexpression correlates with immune evasion phenotypes and resistance to interferon-based therapies. In neurons, altered ADP-ribosylation by PARP11 may affect axonal transport or synaptic signaling.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
