| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PARP15 recombinant protein (Position: N47-R555) was used as the immunogen for the PARP15 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PARP15 Antibody / Poly ADP-ribose polymerase 15 is a anti-PARP15 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: PARP15
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC/IF, FACS, ELISA
Biological background
PARP15 is encoded by the PARP15 gene located on human chromosome 3q21.1. The protein comprises two N-terminal macro domains, a WWE domain involved in protein�protein interactions, and a C-terminal catalytic PARP domain. PARP15 localizes mainly to the nucleus and cytoplasmic granules, where it may regulate mRNA metabolism and response to stress. It has been implicated in cell survival pathways, as well as in the regulation of transcription factors under conditions of nutrient deprivation or oxidative stress.
Research using the PARP15 antibody has demonstrated its involvement in cancer cell proliferation and inflammation. Aberrant expression of PARP15 has been observed in lymphomas and certain solid tumors, where it may contribute to altered transcriptional control and resistance to apoptosis. Western blot analysis typically detects a band at approximately 74�78 kDa. Immunocytochemical staining shows both nuclear and cytoplasmic localization, depending on cell type and stress conditions. Functional studies suggest that PARP15 can auto-modify and regulate its own enzymatic activity, similar to other PARP family members.
In recent years, PARP15 has attracted attention as part of the broader ADP-ribosylation signaling network, which includes enzymes, hydrolases, and binding proteins that control protein modification during stress responses. The antibody supports exploration of this enzyme's role in RNA granule formation, viral defense, and oncogenic signaling pathways.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
