| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PARP16 recombinant protein (Position: A9-Y254) was used as the immunogen for the PARP16 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PARP16 Antibody / Poly ADP-ribose polymerase 16 is a anti-PARP16 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Cytoplasm (ER).
Key elements and design rationale
- Target: PARP16
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): IHC, FACS, ELISA
Biological background
PARP16 is encoded by the PARP16 gene located on human chromosome 15q21.2. The protein is approximately 38 kilodaltons and uniquely localized to the ER membrane, where it functions as a tail-anchored enzyme with its catalytic domain facing the cytoplasm. Unlike canonical PARPs that catalyze poly(ADP-ribosylation), PARP16 primarily transfers single ADP-ribose moieties to substrate proteins (mono-ADP-ribosylation). This modification modulates the activity of key ER stress sensors, including PERK and IRE1-alpha, enhancing their signaling output during the UPR.
The PARP16 antibody typically detects a 38 kilodalton protein by western blot, and immunofluorescence confirms its ER localization. Functional studies demonstrate that PARP16 activity is induced upon ER stress caused by misfolded protein accumulation or chemical perturbation. Activated PARP16 promotes phosphorylation and activation of PERK and IRE1-alpha, leading to downstream transcriptional responses that restore proteostasis. Inhibition or depletion of PARP16 compromises the UPR, resulting in increased sensitivity to ER stress and enhanced apoptosis.
Beyond its role in ER stress regulation, PARP16 contributes to cancer cell survival and resistance to chemotherapeutic agents that induce proteotoxic stress. Overexpression has been reported in hepatocellular carcinoma, breast cancer, and melanoma, where it supports adaptive stress signaling. Conversely, loss of PARP16 sensitizes cells to stress-induced death, making it a potential therapeutic target.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
