| Field | Specification |
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| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human PASK was used as the immunogen for the PASK antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
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| Target | |
| UniProt # |
Overview
PASK Antibody / PAS kinase is a anti-PASK Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Flow cytometry (FACS) with listed reactivity in Human.
Key elements and design rationale
- Target: PASK
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): FACS
Biological background
PASK is broadly expressed in liver, skeletal muscle, pancreatic islets, and the brain, tissues that exhibit strong metabolic plasticity. In hepatocytes, PASK influences glycogen synthesis and gluconeogenic pathways, while in pancreatic beta cells it modulates insulin secretion. Mice lacking PASK exhibit altered glucose tolerance, reduced hepatic glycogen storage, and impaired mitochondrial function, demonstrating its role in metabolic adaptation. PASK phosphorylates transcriptional coactivators such as PGC-1alpha and CREB-regulated transcriptional coactivator 2 (CRTC2), linking nutrient signaling with gene expression that governs oxidative metabolism.
The PASK antibody is used in metabolic research to detect PASK expression and activation across tissues. Western blotting typically identifies a ~160 kilodalton band corresponding to the full-length kinase, while immunofluorescence localizes it to the cytoplasm and nucleus depending on cell type. In mammalian systems, PASK activity rises during fasting and decreases in nutrient-replete conditions, reflecting its regulatory role in energy balance. Because dysregulation of PASK signaling has been associated with diabetes, obesity, and mitochondrial dysfunction, this antibody provides an important tool for investigating metabolic signaling networks.
Recent studies also implicate PASK in circadian rhythm regulation and differentiation of muscle and stem cells. Its PAS domains act as environmental sensors, adjusting transcriptional programs to oxygen and redox changes. PASK interacts with AMPK and mTOR pathways, highlighting its integration into major metabolic signaling circuits. The PASK antibody enables researchers to explore these regulatory interactions and to characterize PASK as a potential therapeutic target in metabolic disease and aging.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
