| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PAX3 recombinant protein (Position: Q306-E420) was used as the immunogen for the PAX3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PAX3 Antibody / Paired box protein Pax-3 is a anti-PAX3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human. Reported localization: Nuclear.
Key elements and design rationale
- Target: PAX3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, ELISA
Biological background
Functionally, PAX3 antibody identifies a 479-amino-acid transcription factor containing both a paired DNA-binding domain and a homeobox domain. PAX3 regulates genes involved in neural crest formation, muscle lineage specification, and melanocyte development. It acts in concert with cofactors such as SOX10, MITF, and FOXO1 to coordinate developmental gene networks. In muscle precursors, PAX3 activates MyoD and MYF5, driving myogenic commitment and differentiation.
The PAX3 gene is located on chromosome 2q36.1 and is highly expressed in the dorsal neural tube, somites, and limb buds during embryogenesis. It remains active in satellite cells and neural crest-derived lineages in adult tissues. PAX3 activity is modulated by phosphorylation, sumoylation, and interaction with chromatin-remodeling complexes.
Pathologically, mutations in PAX3 cause Waardenburg syndrome types 1 and 3, characterized by hearing loss and pigmentary abnormalities. Chromosomal translocations involving PAX3 (such as PAX3-FOXO1 fusions) drive alveolar rhabdomyosarcoma, a pediatric soft tissue cancer. Aberrant PAX3 signaling also contributes to melanoma progression by enhancing cell migration and survival. Research using PAX3 antibody supports studies in developmental biology, stem cell regulation, and cancer genetics.
PAX3 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect transcription factors in neural and muscle tissues.
Structurally, Paired box protein Pax-3 contains two DNA-binding domains-a paired domain and a homeodomain-linked by a flexible region that allows diverse DNA sequence recognition. The C-terminal transactivation domain mediates recruitment of coactivators and repressors. This antibody enables precise analysis of PAX3's regulatory functions in development and disease.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
