| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human PAX7 was used as the immunogen for the PAX7 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PAX7 Antibody / Paired box protein 7 is a anti-PAX7 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human, Mouse.
Key elements and design rationale
- Target: PAX7
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS
Biological background
PAX7 is encoded by the PAX7 gene located on human chromosome 1p36.13. The protein contains a paired DNA-binding domain, a homeodomain, and a C-terminal transactivation domain. During embryonic development, PAX7 is expressed in neural crest cells and the myogenic progenitor population of the somites. In adults, it marks quiescent muscle satellite cells responsible for postnatal muscle repair and regeneration.
The PAX7 antibody identifies a 55 kilodalton nuclear protein by western blot and demonstrates strong nuclear staining in satellite cells and developing muscle fibers. Loss of PAX7 disrupts myogenic lineage specification, resulting in impaired muscle growth. Functionally, PAX7 activates myogenic regulatory factors such as MYOD and MYF5 and promotes self-renewal of satellite cells through repression of differentiation pathways.
Beyond muscle tissue, PAX7 contributes to neurogenesis, pituitary development, and craniofacial patterning. Dysregulation of PAX7 is linked to rhabdomyosarcoma, where gene fusions with FOXO1 create oncogenic transcriptional drivers.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
