| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PBX4 recombinant protein (Position: A3-Q355) was used as the immunogen for the PBX4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PBX4 Antibody / Pre-B-cell leukemia transcription factor 4 is a anti-PBX4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse.
Key elements and design rationale
- Target: PBX4
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, ELISA
Biological background
Pre-B-cell leukemia transcription factor 4 contains a conserved homeodomain that binds DNA at specific sequence motifs and an N-terminal domain that mediates protein-protein interactions. It functions both as a transcriptional activator and repressor depending on its cofactors and target promoters. The PBX4 antibody enables visualization of nuclear expression and quantification of PBX4 across tissue types, supporting studies into gene regulatory networks controlling pattern formation and organ differentiation.
PBX family proteins act as cofactors for HOX transcription factors, enhancing DNA-binding specificity and transcriptional activation of developmental genes. PBX4 is expressed in gonadal, skeletal, and neural tissues, where it helps coordinate morphogenesis and cell lineage specification. The PBX4 antibody supports detailed characterization of these expression domains in embryonic and adult tissues. Functional studies suggest PBX4 may also influence germ cell maturation and fertility by regulating genes involved in testis differentiation.
In cancer biology, aberrant PBX expression contributes to oncogenesis by altering transcriptional programs that control proliferation and differentiation. Elevated PBX4 levels have been linked to tumor growth, epithelial-mesenchymal transition, and metastasis in several cancers. The PBX4 antibody enables detection of these expression changes and supports functional studies investigating its role in oncogenic transcriptional regulation. Western blotting and immunohistochemical analysis using this antibody reveal nuclear localization typical of transcription factors.
The PBX4 antibody performs effectively in immunofluorescence, immunohistochemistry, and chromatin immunoprecipitation, providing versatility across molecular and developmental biology applications.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
