| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PCDHA2 recombinant protein (Position: S169-D345) was used as the immunogen for the PCDHA2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PCDHA2 Antibody / Protocadherin alpha 2 is a anti-PCDHA2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: PCDHA2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Structurally, PCDHA2 is a single-pass transmembrane glycoprotein composed of six extracellular cadherin repeats, a transmembrane domain, and a conserved cytoplasmic tail that interacts with intracellular signaling molecules. It belongs to the cadherin superfamily, which includes classical cadherins, desmogleins, and other protocadherin clusters (beta and gamma). The extracellular cadherin domains of PCDHA2 mediate homophilic adhesion through calcium-dependent binding, while the cytoplasmic region connects to the actin cytoskeleton via catenins and other scaffold proteins.
Functionally, PCDHA2 promotes cell adhesion specificity during neuronal development. It participates in establishing unique neuronal surface codes that guide synaptic pairing and axonal targeting. In mature neurons, PCDHA2 contributes to dendritic self-avoidance, ensuring that neurites from the same cell do not form redundant connections. It also supports synaptic stability by maintaining adhesion at postsynaptic densities. Co-localization studies show PCDHA2 localized to dendritic membranes and synaptic junctions, where it interacts with cytoskeletal and signaling partners.
PCDHA2 expression is developmentally regulated, with high levels during neural differentiation and synapse formation. Dysregulation or mutation of protocadherin alpha genes, including PCDHA2, has been associated with autism spectrum disorder, epilepsy, and schizophrenia, highlighting their importance in brain circuit organization. Pathway involvement includes cell adhesion, synaptic signaling, and calcium-dependent cell communication. Isoform diversity among protocadherin alpha genes allows neurons to generate individual identity codes critical for neural wiring precision.
The PCDHA2 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
