| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human PCP2 was used as the immunogen for the PCP2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PCP2 Antibody / Purkinje cell protein 2 is a anti-PCP2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: PCP2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB
Biological background
PCP2 contains a GoLoco motif that enables high-affinity binding to inactive GDP-bound Galpha subunits, acting as a guanine nucleotide dissociation inhibitor (GDI). Through this interaction, it regulates G protein-coupled receptor (GPCR) signaling pathways that affect neuronal excitability and neurotransmitter release. PCP2 expression increases during cerebellar development, coinciding with synapse formation and maturation of Purkinje cells.
The PCP2 antibody is widely used in neurobiology, developmental, and signaling research to investigate cerebellar function, G protein regulation, and neuronal connectivity. Western blot analysis identifies a 17 kilodalton band corresponding to PCP2, while immunohistochemistry shows intense staining in Purkinje cell somata and dendrites, as well as retinal bipolar neurons. This antibody enables precise visualization of neuronal subtypes and signaling dynamics in the cerebellum and retina.
Altered expression of PCP2 has been associated with ataxia, autism spectrum disorders, and visual processing abnormalities, reflecting its role in synaptic signal modulation. The PCP2 antibody provides a sensitive tool for exploring cerebellar physiology and G protein regulation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
