| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PCYOX1 recombinant protein (Position: R101-D452) was used as the immunogen for the PCYOX1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PCYOX1 Antibody / Prenylcysteine oxidase 1 is a anti-PCYOX1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Cytoplasm (lysosome).
Key elements and design rationale
- Target: PCYOX1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
Structurally, PCYOX1 is a 501-amino-acid enzyme of approximately 57 kilodaltons containing a flavin adenine dinucleotide (FAD)-binding domain responsible for oxidative catalysis. It localizes to the endoplasmic reticulum and cytosol, where it associates with membranes to metabolize prenylated cysteine derivatives derived from small GTPases such as Ras and Rho. This activity maintains proper turnover of prenylated signaling proteins and limits accumulation of reactive intermediates that could disrupt cell membranes.
The PCYOX1 antibody is widely used in metabolism, enzymology, and redox biology research to study lipid modification turnover, oxidative regulation, and prenylated protein catabolism. Western blot analysis detects a 57 kilodalton band corresponding to PCYOX1, while immunofluorescence shows cytosolic and reticular staining in metabolically active tissues such as liver and kidney. This antibody provides a reliable tool for assessing enzyme function in lipid processing and oxidative homeostasis.
Functionally, PCYOX1 acts downstream of protein prenylation pathways that modify small GTPases for membrane targeting and signal transduction. By degrading prenylcysteine products, it prevents the accumulation of toxic intermediates and contributes to lipid-derived aldehyde metabolism. PCYOX1 activity is linked to oxidative stress response, with increased expression observed in conditions of lipid peroxidation and metabolic overload. Dysregulation of PCYOX1 expression has been associated with atherosclerosis and metabolic disease, implicating its role in vascular oxidative stress. The PCYOX1 antibody provides a valuable reagent for studying the interplay between protein modification, lipid metabolism, and oxidative signaling.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
