| Field | Specification |
|---|---|
| Formulation | |
| Molecular Weight | |
| Product Type | |
| Shipping | |
| Species | |
| Storage | |
| UniProt # |
Scientific Background
Human secreted Programmed Cell Death 1 (PD-1)-Fc fusion protein, also known as CD279, PDCD1, and SLEB2, GenBank Accession No. NM_014143, a.a. 25-167 with an E136A mutation, fused at the Cterminus with the Fc portion of human IgG1, and C-terminal AviTag™, expressed in a HEK293 cell expression system and enzymatically biotin-labeled using AviTag™ technology. MW = 44.5 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Product Description
Mammalian (HEK293) ExpressionRecombinant PD-1 Human protein is produced using a validated Mammalian (HEK293) expression system and supplied in aqueous buffer solution. Suitable for enzyme kinetics, inhibitor screening, binding assays, structural studies, and related biochemical research applications.
Protein Specifications
| UniProt ID | Q15116 |
|---|---|
| Expression System | Mammalian (HEK293) |
| Amino Acids / Region | 25-167 |
| Molecular Weight | 44.5 kDa |
| Formulation | 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, pH 7.4, and 20% glycerol |
| Storage | At least 6 months at -80°C. |
| Biosafety Level | Not applicable (BSL-1) |
Safety & Handling
Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.
This protein was produced in Mammalian (HEK293). Expression system selection determines post-translational processing, disulfide bond formation, and co-factor incorporation — all of which affect enzymatic activity. Insect cell (Sf9) systems are preferred for kinases and multi-subunit enzymes that require phosphorylation or chaperone assistance; E. coli is used for structurally simpler proteins.
This protein spans amino acids 25-167. Confirm the region includes your domain of interest — the active site, binding pocket, or substrate recognition sequence — before placing your order. Refer to the UniProt database for domain annotation.
Purity is assessed by SDS-PAGE; see the Certificate of Analysis. A gel image is provided with each lot. BPS Bioscience performs rigorous QC on each lot, including purity assessment and functional activity testing where applicable. Contact technical support if purity ≥99% is required for biophysical measurements.
Useful for the study of protein-protein interaction and in vitro and in vivo protein function. Refer to the product datasheet for validated protocols and recommended assay conditions. Contact BioHippo technical support for application-specific guidance.
At least 6 months at -80°C. Avoid repeated freeze-thaw cycles — prepare single-use working aliquots. Add BSA or glycerol to aliquots if storing diluted enzyme is necessary. Typical stability is at least 6 months at −80°C.
BioHippo offers flexible sourcing for qualified research institutions and partners.
- Bulk quantities: Large-scale orders for HTS campaigns or structural studies.
- Custom constructs: Alternative tag positions, truncation variants, or point mutants may be available upon request.
- Biotinylated variants: Avi-Tag site-specific biotinylation is available for SPR/BLI surface capture applications.
- Extended QC data: Activity assay data, SEC-HPLC profiles, or additional purity methods available on request.
Contact BioHippo customer support for custom requirements.
- Freeman, G.J., et al., J. Exp. Med. 2000 Oct 2;192(7):1027-34.
- Dong, H., et al., Nat Med.
- Dec;5(12):1365-9.
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