| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PDC recombinant protein (Position: M1-E246) was used as the immunogen for the PDC antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PDC Antibody / Phosducin is a anti-PDC Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Mouse, Rat.
Key elements and design rationale
- Target: PDC
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
Functionally, PDC antibody identifies a 28 kDa protein that plays a central role in the visual signal transduction cascade. In dark-adapted photoreceptors, phosducin forms a complex with Gbetagamma subunits, preventing reassociation with the alpha subunit and thereby maintaining the inactive G-protein state. Upon light stimulation, elevated cAMP and phosphorylation by protein kinase A (PKA) reduce its affinity for Gbetagamma, freeing these subunits to reassemble with transducin alpha and activate cGMP phosphodiesterase. This mechanism ensures the precise timing and sensitivity of visual signaling.
The PDC gene is located on chromosome 1q25.2 and encodes a protein consisting of a central G-protein beta-binding domain. Beyond the retina, phosducin is expressed in pineal gland, olfactory neurons, and parts of the brain, suggesting broader roles in neuromodulation. It participates in feedback control of G-protein signaling, potentially influencing synaptic plasticity and hormone release. Phosducin is evolutionarily conserved, with homologs present in multiple vertebrate species, highlighting its importance in sensory transduction and neural signaling.
PDC antibody is used in neurobiology and vision research to explore phototransduction mechanisms, G-protein signaling regulation, and phosphorylation dynamics. In rod cells, phosphorylation state changes of phosducin reflect illumination conditions and photoreceptor adaptation. Studies also show that phosducin participates in stress responses by modulating sympathetic nervous system activity, linking retinal signaling pathways with systemic physiological regulation.
Structurally, phosducin comprises a coiled-coil N-terminal region required for Gbetagamma binding and a C-terminal domain that interacts with chaperones and kinases. It is phosphorylated at multiple serine residues, including Ser54 and Ser73, which alter its subcellular distribution and binding capacity. These regulatory mechanisms fine-tune G-protein signaling under varying environmental and metabolic conditions. Mutations or dysregulation of PDC expression have been implicated in retinal degeneration and light adaptation defects.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
