| Field | Specification |
|---|---|
| Alternative Names | 5''-cyclic phosphodiesterase 8A; cAMP specific cyclic nucleotide phosphodiesterase 8A; FLJ16150; High affinity cAMP specific and IBMX insensitive 3'5' cyclic phosphodiesterase 8A; High affinity cAMP-specific and IBMX-insensitive 3''; HsT19550; PDE 8A; PDE8A; PDE8A_HUMAN; Phosphodiesterase 8A; Phosphodiesterase8A; Weakly similar to 3'5' cyclic nucleotide phosphodiesterase |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Recombinant Human High affinity cAMP-specific and IBMX-insensitive 3\',5\'-cyclic phosphodiesterase 8A protein (596-829AA) |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Storage | |
| Target | |
| UniProt # |
Overview
This is a polyclonal anti-PDE8A antibody raised in Rabbit, conjugated with Biotin, with confirmed utility in ELISA. It is designed to detect PDE8A protein in Human and supports researchers working in signal transduction contexts.
Key elements and design rationale
- Immunogen: Recombinant Human High affinity cAMP-specific and IBMX-insensitive 3\',5\'-cyclic phosphodiesterase 8A protein (596-829AA) — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.
- Host species (Rabbit): Requires anti-rabbit-IgG secondary reagents for indirect detection.
- Polyclonal format: Recognizes multiple epitopes for robust signal; inherent lot-to-lot variability requires appropriate controls.
- Isotype (IgG): Matched secondary antibodies and isotype controls required. Compatible with standard Protein A/G purification workflows.
- Purification (Antigen affinity purification): Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.
- Biotin conjugate: Amplified detection via streptavidin/avidin reporters. Block endogenous biotin in biotin-rich tissue samples.
Biological background
PDE8A (also referred to as 5''-cyclic phosphodiesterase 8A, cAMP specific cyclic nucleotide phosphodiesterase 8A, FLJ16150, High affinity cAMP specific and IBMX insensitive 3'5' cyclic phosphodiesterase 8A, High affinity cAMP-specific and IBMX-insensitive 3'', HsT19550, PDE 8A, PDE8A, PDE8A_HUMAN, Phosphodiesterase 8A, Phosphodiesterase8A, Weakly similar to 3'5' cyclic nucleotide phosphodiesterase) is a protein target studied in Human systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of PDE8A biology in signal transduction.
Common research applications
- ELISA: Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.
Notes for experimental interpretation
- Isotype controls: Use an isotype-matched (IgG from Rabbit) control at equivalent concentration to assess non-specific background.
- Cross-reactivity: Polyclonal preparations may cross-react with related proteins. Orthogonal validation (siRNA, KO lysate) is recommended.
- Matrix effects: Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.
- Species reactivity: Confirmed for Human. Extrapolation to untested species requires empirical validation.
This product is a polyclonal antibody purified from rabbit antiserum.
PDE8A is a protein target in Human biology. This polyclonal antibody raised in Rabbit is designed to detect PDE8A in ELISA applications, with IgG isotype.
Reported reactive against Human. Immunogen derived from Human. Cross-reactivity with other species should not be assumed without documented data or empirical testing.
Reported for ELISA. Each format requires independent dilution optimization and appropriate controls. Unlisted applications require empirical testing.
Biotin enables streptavidin/avidin-amplified detection. Block endogenous biotin in biotin-rich tissues.
(1) Isotype control — IgG from Rabbit at matching concentration; (2) Positive control — known PDE8A-expressing cell/tissue; (3) Negative control — knockdown/knockout sample for specificity; (4) Dilution linearity — verify proportional signal decrease in your matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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