| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PDE8A recombinant protein (Position: R32-E495) was used as the immunogen for the PDE8A antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PDE8A Antibody / Phosphodiesterase 8A is a anti-PDE8A Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: PDE8A
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
PDE8A is encoded by the PDE8A gene located on human chromosome 15q25.3. The enzyme is approximately 93 kilodaltons and contains a conserved catalytic domain flanked by unique N-terminal and C-terminal regulatory regions. PDE8A is expressed in various tissues, including adrenal gland, heart, testis, and immune cells, where it contributes to compartmentalized cAMP degradation and pathway specificity. Its high substrate affinity and resistance to standard inhibitors make it a key regulator of localized signaling domains.
The PDE8A antibody detects multiple isoforms ranging from 85-100 kilodaltons on western blot and reveals cytoplasmic and perinuclear staining in immunofluorescence assays. PDE8A associates with A-kinase anchoring proteins (AKAPs) and participates in cAMP microdomain organization, influencing downstream activation of protein kinase A (PKA) and exchange proteins activated by cAMP (EPACs). In immune cells, PDE8A modulates T-cell receptor signaling and cytokine production, contributing to immune tolerance and inflammatory regulation.
Functional studies indicate that PDE8A influences steroidogenesis, insulin secretion, and energy balance by controlling cAMP-mediated transcriptional events. Knockdown or pharmacological inhibition enhances cAMP signaling and affects processes such as sperm motility, cortisol synthesis, and cardiac contractility. Altered PDE8A expression has been linked to autoimmune diseases, infertility, and metabolic disorders.
PDE8A also plays roles in cancer biology, where its dysregulation affects cell proliferation and migration through altered PKA activity and transcriptional responses.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
