| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | PDPK1; PDK1; 3-phosphoinositide-dependent protein kinase 1; hPDK1 |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PDK1. AA range:1-50 |
| Isotype | |
| Product Type | |
| Reactivity | |
| Storage |
Product Overview
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.,PTM:Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,subcellular location:Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.,subunit:Interacts with TUSC4.,tissue specificity:Appears to be expressed ubiquitously.,
Polyclonal Rabbit AntibodyValidated Applications
| Application | Recommended Dilution |
|---|---|
| Western Blot | Refer to datasheet |
| Immunohistochemistry | Refer to datasheet |
| Immunofluorescence | Refer to datasheet |
| ELISA | Refer to datasheet |
Immunogen & Specificity
Immunogen: The antiserum was produced against synthesized peptide derived from human PDK1. AA range:1-50
Subcellular Localization: Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Cell junction, focal adhesion. Tyrosine phosphorylation seems to occur only at the cell membrane. Translocates to the cell membrane following insulin stimulation by a mechanism that involves binding to GRB14 and INSR. SRC and HSP90 promote its localization to the cell membrane. Its nuclear localization is dependent on its association with PTPN6 and its phosphorylation at Ser-396. Restricted to the nucleus in neuronal cells while in non-neuronal cells it is found in the cytoplasm. The Ser-241 phosphorylated form is distributed along the perinuclear region in neuronal cells while in non-neuronal cells it is found in both the nucleus and the cytoplasm. IGF1 transiently increases phosphorylation at Ser-241 of neuronal PDPK1, resul
UniProt/Swiss-Prot: O15530
Product Specifications
| Host Species | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Species Reactivity | Human, Mouse, Rat, Monkey |
| Concentration | 1 mg/ml |
| Observed Band (kDa) | 60kD |
| Storage | -20°C/1 year |
Reactivity Notes
Confirmed reactivity in Human, Mouse, Rat, Monkey samples; validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA.
Safety & Handling
- For Research Use Only (RUO). Not intended for diagnostic procedures.
- Shipping: cold-chain shipment (typically with ice packs).
- Upon receipt: store at recommended temperature as soon as possible.
- Sales terms and conditions: Please review prior to ordering.
Related Products
Explore additional antibodies and related detection reagents for PDHK1 in the BioHippo catalog.
This antibody has been validated for Western Blot, Immunohistochemistry, Immunofluorescence. Refer to the product datasheet for recommended dilutions and protocol guidance for each application.
Refer to the datasheet for recommended dilution ranges per application. Optimal dilution may vary by sample type, fixation method, and detection system — a titration experiment is recommended prior to full experimental use.
This antibody has been validated or predicted to recognize Human, Mouse, Rat, Monkey samples, based on immunogen sequence homology. For unlisted species, contact our team for guidance.
This antibody was raised against a specific peptide or recombinant protein fragment corresponding to PDHK1. Exact immunogen details are provided in the product datasheet.
Conjugated formats may be available upon request. Contact the BioHippo team for custom conjugation options including fluorophore, biotin, or enzyme labeling.
BioHippo can support custom antibody formatting and service options for selected products. Contact us to discuss your project requirements.
Potential customization options include:
- Custom conjugation: fluorophore, biotin, enzyme, or nanoparticle labeling available on request for qualified antibodies.
- Bulk or scale-up quantities: volume pricing and custom lead time estimates available for larger orders.
- Alternative formulations: azide-free, carrier protein-free, or lyophilized formats available upon request for selected products.
- OEM and private-label supply: custom packaging and branding available for commercial applications.
Submit your project specifications via the product contact form or reach us at support@ebiohippo.com for a tailored quotation.
