| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PDLIM4 recombinant protein (Position: Y137-D271) was used as the immunogen for the PDLIM4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PDLIM4 Antibody / PDZ and LIM domain protein 4 is a anti-PDLIM4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: PDLIM4
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, IP, FACS, ELISA
Biological background
PDLIM4 is encoded by the PDLIM4 gene located on human chromosome 5q31.1. The protein is approximately 330 amino acids long and contains a PDZ domain that binds actin-associated proteins and a LIM domain that mediates interactions with kinases and transcriptional regulators. PDLIM4 localizes primarily to actin stress fibers and focal adhesions, where it contributes to cytoskeletal organization and mechanotransduction.
The PDLIM4 antibody detects a 36 kilodalton band by western blot and shows filamentous cytoplasmic staining under immunofluorescence microscopy. Functionally, PDLIM4 serves as a scaffold linking cytoskeletal tension to gene expression pathways involved in differentiation and proliferation. It interacts with alpha-actinin, integrins, and protein kinase C, coordinating mechanical and biochemical signals during cell adhesion and migration.
PDLIM4 also acts as a tumor suppressor and has been reported to be downregulated or epigenetically silenced in several cancers, including prostate and breast carcinoma. Restoration of PDLIM4 expression suppresses cell growth and induces apoptosis, suggesting its role in controlling oncogenic transformation. Beyond cancer, PDLIM4 contributes to osteoblast differentiation and bone remodeling by regulating cytoskeletal integrity and mechanical signaling.
Because of its dual role in cytoskeletal architecture and transcriptional regulation, PDLIM4 serves as a critical link between cellular mechanics and gene expression.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
