| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PDPR recombinant protein (Position: E155-E655) was used as the immunogen for the PDPR antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PDPR Antibody / Pyruvate dehydrogenase phosphatase regulatory subunit is a anti-PDPR Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: PDPR
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
PDPR functions by modulating the catalytic activity of PDP1 and PDP2 phosphatase subunits, facilitating dephosphorylation and reactivation of PDC. This regulation ensures efficient conversion of pyruvate to acetyl-CoA, thereby supporting ATP generation under nutrient-rich conditions. PDPR also influences glucose oxidation rates and metabolic adaptation during fasting, exercise, or insulin stimulation. Co-localization studies show PDPR associating with PDC and PDP1 in the mitochondrial matrix, maintaining dynamic control over carbohydrate flux.
Structurally, PDPR contains an N-terminal domain involved in protein-protein interactions and a C-terminal alpha-helical region required for complex stability. It belongs to the pyruvate dehydrogenase regulatory protein family, which fine-tunes mitochondrial energy metabolism. The protein�s structural motifs enable binding with PDP catalytic subunits and coordination with mitochondrial targeting sequences for correct localization.
Functionally, PDPR integrates metabolic and hormonal cues to maintain energy homeostasis. It plays a vital role in glucose utilization, mitochondrial respiration, and lipid oxidation balance. PDPR is regulated by insulin, glucagon, and nutrient availability, allowing cells to rapidly switch between carbohydrate and fatty acid metabolism. During embryonic development, PDPR supports energy demands of rapidly growing tissues, particularly cardiac and skeletal muscle. Pathway involvement includes the TCA cycle, oxidative phosphorylation, and insulin signaling pathways that coordinate energy metabolism.
Dysregulation of PDPR activity is linked to metabolic diseases, including diabetes, obesity, and mitochondrial disorders. Reduced PDPR function leads to impaired PDC activation, resulting in accumulation of pyruvate and lactic acid, while overactivation can alter glucose oxidation balance. Genetic variants in PDPR have been associated with altered fasting glucose levels and insulin sensitivity. Because of its role in mitochondrial regulation, PDPR has also been studied in cancer metabolism, where metabolic reprogramming supports tumor cell proliferation.
Immunohistochemical staining using PDPR antibody shows mitochondrial localization in liver, cardiac, and muscle cells. The PDPR antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
