| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PDX1 recombinant protein (Position: M1-E145) was used as the immunogen for the PDX1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PDX1 Antibody / Pancreatic and duodenal homeobox 1 is a anti-PDX1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Nuclear.
Key elements and design rationale
- Target: PDX1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, FACS, ELISA
Biological background
Functionally, PDX1 antibody identifies a 283-amino-acid homeodomain-containing protein that binds to promoter regions of multiple pancreatic genes, including insulin, somatostatin, glucokinase, and GLUT2. PDX1 interacts with transcriptional cofactors such as PBX1, NEUROD1, and MAFA to activate insulin transcription and coordinate glucose-stimulated insulin secretion. During embryogenesis, PDX1 marks multipotent progenitors in the foregut endoderm that give rise to all pancreatic cell lineages.
The PDX1 gene is located on chromosome 13q12.2 and is highly expressed in the pancreas, particularly in beta cells of the islets of Langerhans and in the duodenal epithelium. PDX1 expression is regulated by glucose, nutrient status, and signaling pathways including PI3K/AKT and MAPK. Its activity ensures proper glucose homeostasis and metabolic regulation.
Pathologically, mutations in PDX1 cause pancreatic agenesis, maturity-onset diabetes of the young type 4 (MODY4), and contribute to type 2 diabetes through impaired beta-cell function. Loss of PDX1 expression leads to beta-cell dedifferentiation, while overexpression can promote transdifferentiation of non-beta pancreatic cells into insulin-producing phenotypes. Research using PDX1 antibody supports studies in diabetes, pancreatic development, and regenerative medicine.
PDX1 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect transcription factors involved in endocrine development.
Structurally, Pancreatic and duodenal homeobox 1 contains a highly conserved homeobox domain responsible for DNA binding and nuclear localization signals that target it to the nucleus. Post-translational modifications such as phosphorylation modulate PDX1 stability and transcriptional activity. This antibody enables investigation of PDX1's role in pancreatic morphogenesis, beta-cell identity maintenance, and metabolic gene regulation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
