| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived mouse PDZK1 recombinant protein (Position: R8-R502) was used as the immunogen for the PDZK1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PDZK1 Antibody / PDZ domain-containing 1 / NHERF3 is a anti-PDZK1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: PDZK1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
Functionally, PDZK1 antibody identifies a 519-amino-acid cytoplasmic protein containing four PDZ domains that mediate multiprotein complex formation at the apical membrane. PDZK1 anchors transporters such as the sodium-hydrogen exchanger NHE3 and the scavenger receptor SR-BI, facilitating ion exchange and lipid uptake. It coordinates interactions between membrane proteins and signaling molecules that maintain epithelial polarity and transport efficiency.
The PDZK1 gene is located on chromosome 1q21.1 and is highly expressed in kidney, liver, and intestine. In hepatocytes, PDZK1 regulates cholesterol homeostasis through its interaction with SR-BI, while in renal and intestinal epithelia it modulates absorption and reabsorption processes. PDZK1's scaffolding role ensures efficient communication between transporters and cytoskeletal components.
Pathologically, dysregulated PDZK1 expression is linked to disorders of lipid metabolism, hypertension, and cancer. Reduced PDZK1 can impair high-density lipoprotein (HDL) uptake and disrupt cholesterol transport. Overexpression has been associated with tumor progression through altered signaling dynamics. Research using PDZK1 antibody enhances understanding of epithelial transport regulation and metabolic signaling.
PDZK1 antibody is suitable for western blotting, immunofluorescence, and immunohistochemistry to detect PDZK1 in epithelial tissues and cultured cells.
Structurally, PDZK1 features four PDZ domains arranged to allow simultaneous binding of multiple partners, forming scaffolded microdomains at the plasma membrane. This antibody enables analysis of PDZK1-mediated protein complexes that maintain cellular polarity and metabolic balance.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
