| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human PECR was used as the immunogen for the PECR antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PECR Antibody / Peroxisomal trans-2-enoyl-CoA reductase is a anti-PECR Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF) with listed reactivity in Human, Rat. Reported localization: Cytoplasm (Peroxisome).
Key elements and design rationale
- Target: PECR
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF
Biological background
Functionally, PECR antibody identifies a 303-amino-acid peroxisomal matrix enzyme encoded by the PECR gene, which contributes to the reduction step of unsaturated fatty acid metabolism. PECR participates in both peroxisomal beta-oxidation and fatty acid elongation cycles, converting trans-2-enoyl-CoA substrates into saturated acyl-CoA derivatives for further processing. It plays a critical role in maintaining normal lipid composition within cellular membranes and is essential for the metabolism of very long-chain and polyunsaturated fatty acids.
The PECR gene is located on chromosome 2q35 and is expressed in liver, kidney, brain, and skeletal muscle. Its expression is regulated by peroxisome proliferator-activated receptors (PPARs), linking it to lipid metabolism and oxidative stress responses. The enzyme's localization to peroxisomes allows it to cooperate with other beta-oxidation enzymes such as ACOX1 and HSD17B4.
Pathologically, PECR dysfunction or mutation has been associated with metabolic abnormalities and neurodegenerative disease. Variants in PECR can lead to abnormal lipid accumulation and impaired energy metabolism, contributing to disorders such as Refsum-like disease and retinal dystrophy. Research using PECR antibody supports studies in lipid metabolism, peroxisomal function, and oxidative stress regulation.
PECR antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect peroxisomal enzymes.
Structurally, Peroxisomal trans-2-enoyl-CoA reductase belongs to the short-chain dehydrogenase/reductase (SDR) family and contains conserved catalytic motifs (TGxxxGxG and YxxxK) required for NADPH binding and substrate reduction. The enzyme forms homotetramers within peroxisomes and contributes to redox balance via NADPH utilization. This antibody allows investigation of PECR's role in peroxisomal metabolism, energy balance, and cellular redox homeostasis.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
