| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PEPD recombinant protein (Position: R33-K493) was used as the immunogen for the PEPD antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PEPD Antibody / Peptidase D is a anti-PEPD Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: PEPD
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, FACS, ELISA
Biological background
PEPD is encoded by the PEPD gene located on human chromosome 19q13.11. The protein is approximately 493 amino acids long and requires manganese as a cofactor for catalytic activity. PEPD functions as a homodimer, with each subunit contributing to the active site. It is expressed in a wide range of tissues, particularly in the liver, kidney, and fibroblasts, where it facilitates collagen metabolism and amino acid homeostasis.
The PEPD antibody detects a 54 kilodalton band by western blot and shows cytosolic distribution under immunofluorescence microscopy. PEPD participates in proline recycling for collagen resynthesis, making it essential for connective tissue maintenance and wound healing. Mutations in PEPD cause prolidase deficiency, a rare metabolic disorder characterized by skin ulcers, developmental delay, and recurrent infections due to impaired collagen degradation and imbalanced proline metabolism.
In addition to its metabolic role, PEPD modulates cell signaling by acting as an extracellular ligand for epidermal growth factor receptor (EGFR), activating proliferative and survival pathways in response to tissue injury. It also influences immune responses by regulating proline-derived metabolites that affect macrophage activation and oxidative balance.
Because PEPD connects collagen metabolism, cell signaling, and tissue repair, it serves as an important target in research on fibrosis, wound healing, and metabolic disorders.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
