Peroxidase Conjugated Affinity Purified anti-Rat IgG [H&L] [Donkey]

SKU:BHA10908407
Suppliers
Applied Biological Materials (abm) Inc.
Applied Biological Materials (abm) Inc.
Details Products
Overview
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Anti-Peroxidase Conjugated Affinity Purified anti-Rat IgG [H&L] [Donkey] antibody. Reactive against Rat targets.
Species Reactivity Rat
Options selector
Catalog no. Size
SH027 250 ug
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 250 ug
  • Lead time: In Stock at Manufacturer options listed in “Availability Content”; other statuses may take longer.
  • Storage: This product is stable for several weeks at 4° C as an undiluted liquid. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at −20°C as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Mfr No SH027
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Immunogen Rat IgG whole molecule
Product Type
  • Primary Antibody
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Rat IgG and Rat Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.
Reactivity
  • Rat
Shipping Ice Pack
Storage This product is stable for several weeks at 4° C as an undiluted liquid. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.

Product Overview

Research Use Only

Minimum Cross reactivity to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins

Validated Applications

Application data not specified. Contact us for validation guidance.

Key Specifications

Species Reactivity Rat
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Catalog No. SH027

Immunogen / Antigen

Rat IgG whole molecule

Reactivity Notes

This antibody has been validated for reactivity against Rat targets. Cross-reactivity with other species may exist but has not been formally established.

Additional Information

Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA), IgG and Protease free

Storage & Handling

This product is stable for several weeks at 4° C as an undiluted liquid. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.

Safety

For Research Use Only (RUO). Not intended for diagnostic, therapeutic, or veterinary use. Handle according to standard laboratory biosafety guidelines. Avoid skin and eye contact. Dispose of waste in accordance with local regulations.

What applications has the Peroxidase Conjugated Affinity Purified anti-Rat IgG [H&L] [Donkey] been validated for?

This antibody has been validated for: please consult the datasheet. For each application, follow the recommended dilutions on the product datasheet and optimize empirically for your sample type.

What is the recommended dilution range?

This product has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:80,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher.

Which species does this antibody recognize?

This antibody has been confirmed reactive against: Rat. Cross-reactivity with other species may occur due to sequence homology but is not guaranteed without independent validation.

What is the immunogen / antigen used to generate this antibody?

Rat IgG whole molecule

How should this antibody be stored and handled?

This product is stable for several weeks at 4° C as an undiluted liquid. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration. Avoid repeated freeze-thaw cycles. Centrifuge vials briefly before opening. Dilute immediately before use.

Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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