| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PEX1 recombinant protein (Position: Q256-R1266) was used as the immunogen for the PEX1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PEX1 Antibody / Peroxisome biogenesis factor 1 is a anti-PEX1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: PEX1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, FACS, ELISA
Biological background
PEX1 contains two ATPase domains characteristic of the AAA+ protein family and forms a complex with PEX6 that attaches to the peroxisomal membrane via PEX26. This complex functions as an energy-dependent disassembly machine, resetting the import apparatus after cargo delivery. Proper PEX1 activity is essential for peroxisome maintenance and metabolic homeostasis, including beta-oxidation of very long-chain fatty acids and plasmalogen synthesis.
The PEX1 antibody is widely used in cell biology, metabolism, and genetic disease research to study peroxisomal biogenesis, import machinery, and metabolic regulation. Western blot analysis identifies a 147 kilodalton band corresponding to PEX1, while immunofluorescence shows punctate cytoplasmic staining consistent with peroxisomal localization. This antibody provides a sensitive tool for investigating peroxisomal function and organelle dynamics.
Mutations in PEX1 are the most common cause of Zellweger spectrum disorders, a group of peroxisomal biogenesis disorders leading to severe metabolic dysfunction. Loss of PEX1 disrupts enzyme import, leading to defective peroxisome assembly and accumulation of toxic lipid intermediates. The PEX1 antibody supports studies of peroxisome biology, organelle biogenesis, and genetic metabolic disease.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
