| Field | Specification |
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| Host | |
| Immunogen | A synthesized peptide derived from human PEX11B was used as the immunogen for the PEX11B antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PEX11B Antibody / Peroxisomal membrane protein 11B is a anti-PEX11B Rabbit antibody Recombinant Rabbit Monoclonal clone 29P66 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human, Mouse. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: PEX11B
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 29P66, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC
Biological background
PEX11B antibody is widely applied in cell biology, metabolism, and disease research. By detecting PEX11B, researchers can explore how peroxisomes multiply and adapt to metabolic demands. Knockout studies show that PEX11B deficiency leads to reduced peroxisome number, altered lipid metabolism, and developmental abnormalities. This highlights its importance in peroxisome biogenesis disorders.
Applications include western blotting, immunohistochemistry, immunofluorescence, and ELISA. Western blotting identifies PEX11B protein in peroxisome enriched fractions, immunohistochemistry maps tissue distribution in liver and kidney, and immunofluorescence highlights peroxisomal membranes. These assays provide robust tools for evaluating peroxisome biology in health and disease.
Mutations in PEX11B cause peroxisome biogenesis disorders characterized by cataracts, intellectual disability, and developmental delay. These disorders illustrate the essential role of PEX11B in organelle maintenance. By applying PEX11B antibody, scientists can investigate peroxisomal dynamics, inheritance, and disease mechanisms. In addition, altered peroxisome function is linked to metabolic syndromes, cancer, and neurodegeneration, making PEX11B relevant beyond rare genetic disease.
PEX11B interacts with dynamin like proteins and other peroxisome fission factors, coordinating peroxisome proliferation with mitochondrial dynamics. This crosstalk underscores its importance in cellular energy balance.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
