| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PEX19 recombinant protein (Position: Q51-A269) was used as the immunogen for the PEX19 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PEX19 Antibody / Peroxisomal biogenesis factor 19 is a anti-PEX19 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: PEX19
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Functionally, PEX19 antibody recognizes a 299-amino-acid protein that binds newly synthesized PMPs in the cytosol, shielding them from aggregation and misfolding. PEX19 delivers these PMPs to the peroxisomal membrane by interacting with the docking protein PEX3, which anchors them into the membrane bilayer. This process is vital for peroxisomal membrane protein biogenesis, division, and inheritance. PEX19 functions both as a chaperone and an import receptor, playing a dual role in organelle biogenesis.
The PEX19 gene is located on chromosome 1q22 and encodes a soluble protein that cycles between cytosolic and peroxisomal compartments. It contains a C-terminal farnesylation motif that anchors it transiently to membranes, enhancing interaction with peroxisomal receptors. Mutations in PEX19 cause peroxisome biogenesis disorder type 14B (Zellweger spectrum disorder), resulting in defective peroxisome formation and metabolic dysfunction characterized by accumulation of very-long-chain fatty acids and bile acid intermediates.
In healthy cells, PEX19 interacts with multiple peroxins, including PEX3 and PEX16, forming a network that ensures correct targeting and integration of PMPs. Its function is tightly coordinated with matrix protein import, supporting peroxisomal maintenance and proliferation. Beyond peroxisome assembly, PEX19 may influence lipid metabolism and antioxidant defense through its role in peroxisomal homeostasis. Dysregulation of PEX19 expression contributes to oxidative stress and metabolic imbalance.
PEX19 antibody is used in cell biology, biochemistry, and metabolism research to study peroxisomal formation and dynamics. It supports applications such as immunoblotting, immunofluorescence, and organelle isolation to evaluate peroxisome abundance, protein import, and morphology. The antibody is especially valuable for identifying peroxisomal defects in metabolic and neurodegenerative diseases. In hepatocytes and fibroblasts, PEX19 localization provides a sensitive indicator of peroxisome assembly status.
Structurally, PEX19 contains multiple tetratricopeptide repeat (TPR)-like motifs that mediate binding to PMPs. Its N-terminal region recognizes hydrophobic transmembrane segments, while the C-terminal farnesylation site facilitates membrane targeting.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
