| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PFDN2 recombinant protein (Position: K18-Q98) was used as the immunogen for the PFDN2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PFDN2 Antibody / Prefoldin subunit 2 is a anti-PFDN2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Rat. Reported localization: Nucleus, Cytoplasm, Mitochondria.
Key elements and design rationale
- Target: PFDN2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
By stabilizing actin and tubulin subunits, PFDN2 supports cytoskeletal organization, cell division, and intracellular transport. Its role as a chaperone subunit is central to maintaining proteostasis, allowing cells to manage misfolded proteins during stress conditions. PFDN2 expression is ubiquitous and tightly regulated to sustain cytoskeletal dynamics across tissues, particularly in neurons, epithelial cells, and fibroblasts.
Structurally, PFDN2 forms one arm of the jellyfish-shaped prefoldin complex, which consists of six subunits (PFDN1-6) arranged in a heterohexameric structure. This configuration provides flexibility for binding diverse substrates. PFDN2 contains coiled-coil and beta-hairpin motifs that mediate interactions with unfolded protein substrates, helping maintain their solubility until delivery to the CCT complex. Its conservation across eukaryotes highlights its fundamental role in protein quality control and cellular stability.
PFDN2 also participates in transcriptional regulation and cellular stress responses, possibly through nuclear interactions under certain conditions. It functions within the broader protein folding and proteostasis network, cooperating with heat shock proteins such as HSP70 and chaperonins to maintain a balanced proteome. Disruption of PFDN2 or other prefoldin subunits leads to cytoskeletal instability, reduced cell proliferation, and heightened sensitivity to proteotoxic stress.
Dysregulation of PFDN2 expression has been linked to several diseases. Reduced prefoldin activity can contribute to neurodegenerative disorders where protein misfolding is pathogenic, such as Parkinson's and Huntington's disease. Overexpression of PFDN2 has been noted in certain cancers, including hepatocellular and colorectal carcinoma, where enhanced folding capacity may support tumor cell growth and resistance to stress. PFDN2 operates in the protein folding and cytoskeletal assembly pathways, underscoring its role in maintaining cell shape and function.
Immunohistochemical staining using PFDN2 antibody demonstrates cytoplasmic localization consistent with its chaperone activity. The PFDN2 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
