| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PFKM recombinant protein (Position: R39-V780) was used as the immunogen for the PFKM antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PFKM Antibody / Phosphofructokinase muscle type is a anti-PFKM Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: PFKM
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, IF, IHC, ICC, WB, FACS
Biological background
Functionally, PFKM antibody identifies a 780-amino-acid cytosolic enzyme that forms homo- or heterotetramers with other phosphofructokinase isoforms (PFKL and PFKP). PFKM activity is allosterically regulated by ATP, citrate, AMP, and fructose-2,6-bisphosphate, allowing fine-tuned control of glycolytic flux according to cellular energy status. Its catalytic action commits glucose to the glycolytic pathway, linking energy demand with carbohydrate metabolism.
The PFKM gene is located on chromosome 12q13.11 and is predominantly expressed in skeletal and cardiac muscle. It plays a vital role in maintaining muscle energy supply during contraction. PFKM expression and activity adapt to metabolic cues, hypoxia, and hormonal regulation, integrating glycolysis with systemic energy homeostasis.
Pathologically, mutations in PFKM cause Glycogen storage disease type VII (Tarui disease), characterized by exercise intolerance, muscle cramps, and myoglobinuria. Deficiency leads to impaired glycolytic ATP production and accumulation of glycogen in muscle fibers. Aberrant PFKM regulation is also associated with metabolic disorders and tumor glycolysis (Warburg effect). Research with PFKM antibody supports studies in metabolism, enzymology, and inherited myopathies.
PFKM antibody is suitable for western blotting, immunohistochemistry, and enzyme assays to detect muscle phosphofructokinase expression.
Structurally, Phosphofructokinase, muscle type forms a tetrameric enzyme with alternating catalytic and regulatory domains. Allosteric binding sites modulate its conformational state and enzymatic efficiency. This antibody allows detailed analysis of PFKM's contribution to energy metabolism and metabolic control.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
