| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PGAM5 recombinant protein (Position: D63-L252) was used as the immunogen for the PGAM5 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PGAM5 Antibody / Phosphoglycerate mutase family member 5 is a anti-PGAM5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Mitochondria.
Key elements and design rationale
- Target: PGAM5
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
Functionally, PGAM5 antibody identifies a 289-amino-acid mitochondrial protein localized to the outer mitochondrial membrane. PGAM5 acts downstream of the MAP3K5-ASK1 signaling axis, dephosphorylating substrates that mediate programmed necrosis and mitophagy. It interacts with proteins such as KEAP1, DRP1, and BCL-XL, influencing mitochondrial fission, redox homeostasis, and apoptosis regulation. By modulating DRP1 phosphorylation, PGAM5 facilitates mitochondrial fragmentation during stress-induced cell death and promotes removal of damaged mitochondria through autophagic processes.
The PGAM5 gene is located on chromosome 12q24.33 and is expressed in metabolically active tissues, including liver, brain, and skeletal muscle. Through its phosphatase activity, PGAM5 integrates oxidative stress signals with mitochondrial quality control, acting as a central node in determining cell fate under energy or redox imbalance.
Pathologically, dysregulation of PGAM5 contributes to neurodegenerative diseases, ischemia-reperfusion injury, and cancer. In neurons, excessive PGAM5 activation leads to mitochondrial fragmentation and cell death, while reduced expression impairs mitophagy and promotes accumulation of damaged mitochondria. In cancer cells, PGAM5 modulates metabolism and apoptosis sensitivity, linking mitochondrial signaling to tumor progression. Research using PGAM5 antibody supports studies in mitochondrial biology, apoptosis, and stress adaptation pathways.
PGAM5 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect mitochondrial phosphatases and regulators of cell death.
Structurally, Phosphoglycerate mutase family member 5 contains a C-terminal catalytic domain with a histidine phosphatase motif (His-Arg-Ser) and an N-terminal transmembrane anchor that tethers it to the outer mitochondrial membrane. The active site structure enables PGAM5 to recognize phosphorylated substrates associated with mitochondrial fission machinery. This antibody facilitates exploration of PGAM5's role in mitochondrial remodeling, necroptosis, and energy metabolism.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
