| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PGLYRP2 recombinant protein (Position: D198-Q524) was used as the immunogen for the PGLYRP2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PGLYRP2 Antibody / Peptidoglycan recognition protein 2 is a anti-PGLYRP2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: PGLYRP2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
PGLYRP2 is secreted primarily by the liver into the bloodstream, where it functions as a circulating peptidoglycan hydrolase. It neutralizes proinflammatory bacterial components, preventing systemic immune activation. In the gut, PGLYRP2 maintains microbiota balance by degrading bacterial fragments that would otherwise trigger toll-like receptor signaling. Genetic studies have associated PGLYRP2 variants with inflammatory bowel disease and asthma, highlighting its role in mucosal immune tolerance. The protein's enzymatic activity also protects against bacterial sepsis by reducing peptidoglycan-induced cytokine production.
The PGLYRP2 antibody is widely used in immunology, microbiology, and liver biology research to study antimicrobial defense and immune modulation. Western blot analysis typically identifies a 62 kilodalton band corresponding to full-length PGLYRP2, while immunohistochemistry reveals cytoplasmic and secretory vesicle localization in hepatocytes and epithelial cells. This antibody supports characterization of innate immune pathways and microbiota interactions. Dysregulation of PGLYRP2 expression can lead to excessive inflammation or increased bacterial susceptibility.
Mechanistically, PGLYRP2 differs from other PGRPs by possessing amidase enzymatic activity rather than bactericidal or signaling roles. It cooperates with PGLYRP1, PGLYRP3, and PGLYRP4 in forming an integrated host defense network.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
