| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PGM1 recombinant protein (Position: M1-Q550) was used as the immunogen for the PGM1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PGM1 Antibody / Phosphoglucomutase 1 is a anti-PGM1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: PGM1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
PGM1 is encoded by the PGM1 gene on human chromosome 1p31.3. It belongs to the phosphoglucomutase family, which includes several isoforms with distinct tissue distributions. The protein is composed of four structural domains forming a catalytic cleft that coordinates substrate binding and phosphoryl transfer. PGM1 deficiency is an inherited metabolic disorder characterized by impaired glycogen metabolism, hypoglycemia, and variable involvement of muscle and liver function. Mutations in the gene lead to partial or complete loss of enzyme activity, resulting in congenital disorder of glycosylation type It (PGM1-CDG).
The PGM1 antibody is commonly used in western blot and immunohistochemistry to detect the 61-63 kDa cytosolic protein. Expression studies demonstrate high levels in metabolically active tissues, including skeletal muscle, cardiac muscle, and hepatocytes. Immunofluorescence assays localize PGM1 primarily to the cytoplasm, consistent with its role in glucose metabolism. Functional studies have linked PGM1 activity to the regulation of glycogenolysis and the maintenance of glucose flux during fasting or exercise. Because of its importance in energy homeostasis, PGM1 serves as a biomarker for metabolic adaptation and carbohydrate-processing disorders.
PGM1 interacts with glycolytic enzymes and participates in metabolic channeling, facilitating efficient substrate transfer within enzymatic complexes. Recent work has shown that PGM1 expression may change in response to hypoxia, oxidative stress, and insulin signaling.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
