| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human PGM1 was used as the immunogen for the PGM1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PGM1 Antibody / Phosphoglucomutase 1 is a anti-PGM1 Rabbit antibody Recombinant Rabbit Monoclonal clone 30P21 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: PGM1
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30P21, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, ICC, IF, IP
Biological background
Phosphoglucomutase 1 functions as a critical branch-point enzyme, controlling the flux of glucose molecules between storage and utilization pathways. Research using PGM1 antibody has shown that its activity is essential for maintaining blood glucose levels during fasting and feeding cycles. The enzyme is expressed in most tissues, but is particularly abundant in liver and muscle, where it helps balance glycogen reserves with immediate energy needs. This makes Phosphoglucomutase 1 indispensable for metabolic flexibility.
Studies with PGM1 antibody have revealed that deficiency in this enzyme leads to PGM1 deficiency, also known as glycogen storage disease type XIV. Patients present with hypoglycemia, hepatomegaly, exercise intolerance, and sometimes congenital disorders of glycosylation, as the enzyme also contributes to protein glycosylation pathways. Mutations in PGM1 impair enzymatic activity, demonstrating the broad role of this protein in both energy metabolism and protein processing.
In cancer research, Phosphoglucomutase 1 has gained attention as a metabolic regulator. Studies with PGM1 antibody have shown that altered expression levels can reprogram glucose metabolism to support rapid tumor growth. By increasing glycolytic flux and maintaining biosynthetic precursors, PGM1 supports cell proliferation. This positions it as a potential biomarker for metabolic adaptation in cancer.
PGM1 antibody is widely used in western blotting, immunohistochemistry, and immunofluorescence. Western blotting quantifies protein levels and detects isoforms, immunohistochemistry reveals tissue distribution in metabolic organs, and immunofluorescence highlights cytoplasmic localization associated with glycogen granules. These experimental applications make PGM1 antibody valuable in basic and clinical research.
By supplying validated PGM1 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
