| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PHF8 recombinant protein (Position: H108-D946) was used as the immunogen for the PHF8 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PHF8 Antibody / Plant homeodomain finger protein 8 is a anti-PHF8 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Nuclear.
Key elements and design rationale
- Target: PHF8
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
PHF8 is encoded by the PHF8 gene located on human chromosome Xp11.22. The protein is approximately 1,024 amino acids in length and contains two major domains: a plant homeodomain (PHD) finger that recognizes trimethylated histone H3 lysine 4 and a Jumonji C (JmjC) catalytic domain responsible for demethylation. By binding to active chromatin marks via the PHD domain, PHF8 is recruited to promoter regions where it removes repressive histone marks, facilitating transcriptional initiation.
The PHF8 antibody detects a 120-140 kilodalton protein by western blot and shows nuclear localization under immunofluorescence microscopy. PHF8 participates in multiple developmental processes, including craniofacial morphogenesis and neural differentiation. In neurons, it regulates genes required for synaptic growth, neurite extension, and neurotransmission. Loss of PHF8 function leads to intellectual disability, cleft lip and palate, and X-linked mental retardation syndromes, emphasizing its critical role in human development.
At the molecular level, PHF8 acts as both an epigenetic activator and repressor, depending on context. It associates with transcription factors such as TCF3 and ZNF711 to coordinate developmental gene expression programs. PHF8 also interacts with components of the RNA polymerase II complex, linking histone demethylation to active transcription elongation. In cancer biology, overexpression of PHF8 enhances cell proliferation and epithelial-to-mesenchymal transition, while its depletion suppresses tumor growth by re-establishing chromatin repression.
Because of its dual functions in transcriptional activation and chromatin organization, PHF8 serves as an important biomarker for studying epigenetic mechanisms in development and disease.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
