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Compound Overview
PHOP (CAS 288862-83-9) is a research-grade small molecule supplied by TargetMol.
Physical Properties
| CAS Number | 288862-83-9 |
|---|---|
| Molecular Formula | C18H18N2O2 |
| Molecular Weight | 294.35 g/mol |
| SMILES | O=C(CCCCCc1ccccc1)c1nc2ncccc2o1 |
| Form | Solid |
| Shipping | Room Temperature (RT) |
Biological Activity
Fatty acid amide hydrolase (FAAH), an enzyme responsible for the hydrolysis and inactivation of fatty acid amides like anandamide and oleamide, has been identified as a target by the potent FAAH inhibitor PHOP. PHOP demonstrates remarkable inhibitory activity with K_i values as low as 0.094 nM for human FAAH and 0.2 nM for rat FAAH. Additionally, through a proteomics assay focusing on the serine hydrolase enzyme family, to which FAAH belongs, PHOP's selectivity was evaluated, presenting IC_50 values of 1.1 nM against FAAH, 1.4 nM against triacylglycerol hydrolase (TGH), and greater than 100 uM against an uncharacterized hydrolase (KIAA1363). This specificity profile of PHOP underscores its potential for yielding precise outcomes in studies involving complex biological systems.
Safety & Regulatory
For Research Use Only (RUO). Not for therapeutic, diagnostic, or clinical use. Wear appropriate PPE and consult the Safety Data Sheet (SDS) prior to handling.
When solubility is listed as < 1 mg/mL, the compound dissolves to less than 1 mg per milliliter in water at 25 °C, pH 7.4. Such compounds require an organic co-solvent such as DMSO to prepare a concentrated stock, which is then diluted into aqueous assay buffer. For cell-based assays, keep the final DMSO concentration below 0.1% (v/v) to avoid solvent-induced cytotoxicity.
Centrifuge the closed vial at 3000 rpm for 1–2 minutes to consolidate the powder at the bottom. For electrostatically charged powders, briefly warm the vial to room temperature and allow it to equilibrate before opening. Then add the appropriate solvent directly onto the powder and vortex before sonicating. Avoid tapping the vial vigorously, as this can generate fine powder aerosol.
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- Add-on labels & handles: D/¹³C/¹⁵N isotopes (LC-MS/internal standards), azide/alkyne or other functional handles for conjugation
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