| Field | Specification |
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| Host | |
| Immunogen | A synthesized peptide derived from human Phospho-AS160 (T642) was used as the immunogen for the Phospho-AS160 (Thr642) antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Phospho-AS160 (Thr642) Antibody / TBC1D4 is a anti-AS160 (phospho-Thr642) Rabbit antibody Recombinant Rabbit Monoclonal clone 31T47 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.
Key elements and design rationale
- Target: AS160 (phospho site: Thr642)
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31T47, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
Phospho-AS160 (Thr642) antibody is widely applied in metabolism, endocrinology, and diabetes research. Detecting phosphorylation at Thr642 provides a direct readout of insulin-stimulated Akt signaling and GLUT4 trafficking. This makes the antibody valuable for studies of insulin resistance, obesity, and type 2 diabetes. In addition, TBC1D4 is expressed in other tissues, linking it to energy homeostasis beyond classical insulin-responsive cells.
Applications for Phospho-AS160 (Thr642) antibody include western blotting, immunohistochemistry, immunofluorescence, and ELISA. Western blot assays detect phosphorylation changes in response to insulin or exercise, immunohistochemistry maps expression in metabolic tissues, and immunofluorescence highlights vesicle trafficking events. These methods allow researchers to connect molecular signaling to physiological glucose uptake.
Dysregulation of TBC1D4 phosphorylation is linked to insulin resistance and diabetes. Reduced phosphorylation impairs GLUT4 trafficking, while mutations in TBC1D4 cause severe postprandial hyperinsulinemia and muscle glycogen storage abnormalities. By applying Phospho-AS160 (Thr642) antibody, scientists can study both common and rare metabolic disorders.
Beyond metabolism, TBC1D4 regulates vesicle trafficking in immune cells, neurons, and other systems. Phosphorylation-dependent regulation highlights its integration with Akt signaling in multiple contexts.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Quantifying post-translational regulation (including phosphorylation) alongside total protein levels.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Phospho-site readouts are condition-dependent and are often compared to total target levels when available.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
