| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Aa2-050; AU019800; AU021329; AU043350; AW146323; BUB 3; BUB 3L; BUB3 (budding uninhibited by benzimidazoles 3; yeast) homolog; bub3; BUB3 budding uninhibited by benzimidazoles 3 homolog; BUB3 mitotic checkpoint protein; BUB3_HUMAN; BUB3L; Budding uninhibited by benomyl; Budding uninhibited by benzimidazoles 3; Budding uninhibited by benzimidazoles 3 homolog (S. cerevisiae); Budding uninhibited by benzimidazoles 3 homolog (yeast); Budding uninhibited by benzimidazoles 3 homolog; Budding uninhibited by benzimidazoles 3; S. cerevisiae; homolog of; C78067; hBUB3; Mitotic checkpoint component; Mitotic checkpoint protein bub3; OTTHUMP00000020679; OTTHUMP00000020680; WD repeat type I transmembrane protein A72.5 |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Peptide sequence around phosphorylation site of Tyrosine 207 (V-E-Y(p)-L-D) derived from Human Bub3. |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Storage | |
| Target | |
| UniProt # |
Overview
This is a polyclonal anti-BUB3 antibody raised in Rabbit, with confirmed utility in ELISA, WB. It is designed to detect BUB3 protein in Human, Mouse, Rat and supports researchers working in others contexts.
Key elements and design rationale
- Immunogen: Peptide sequence around phosphorylation site of Tyrosine 207 (V-E-Y(p)-L-D) derived from Human Bub3. — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.
- Host species (Rabbit): Requires anti-rabbit-IgG secondary reagents for indirect detection.
- Polyclonal format: Recognizes multiple epitopes for robust signal; inherent lot-to-lot variability requires appropriate controls.
- Isotype (IgG): Matched secondary antibodies and isotype controls required. Compatible with standard Protein A/G purification workflows.
- Purification (Antigen affinity purification): Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.
Biological background
BUB3 (also referred to as Aa2-050, AU019800, AU021329, AU043350, AW146323, BUB 3, BUB 3L, BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog, bub3, BUB3 budding uninhibited by benzimidazoles 3 homolog, BUB3 mitotic checkpoint protein, BUB3_HUMAN, BUB3L, Budding uninhibited by benomyl, Budding uninhibited by benzimidazoles 3, Budding uninhibited by benzimidazoles 3 homolog (S. cerevisiae), Budding uninhibited by benzimidazoles 3 homolog (yeast), Budding uninhibited by benzimidazoles 3 homolog, Budding uninhibited by benzimidazoles 3, S. cerevisiae, homolog of, C78067, hBUB3, Mitotic checkpoint component, Mitotic checkpoint protein bub3, OTTHUMP00000020679, OTTHUMP00000020680, WD repeat type I transmembrane protein A72.5) is a protein target studied in Human systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of BUB3 biology in others.
Common research applications
- ELISA: Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.
- WB: Confirms target molecular weight and distinguishes isoforms under denaturing conditions.
Notes for experimental interpretation
- Isotype controls: Use an isotype-matched (IgG from Rabbit) control at equivalent concentration to assess non-specific background.
- Cross-reactivity: Polyclonal preparations may cross-react with related proteins. Orthogonal validation (siRNA, KO lysate) is recommended.
- Matrix effects: Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.
- Species reactivity: Confirmed for Human, Mouse, Rat. Extrapolation to untested species requires empirical validation.
This product is a polyclonal antibody purified from rabbit antiserum.
BUB3 is a protein target in Human, Mouse, Rat biology. This polyclonal antibody raised in Rabbit is designed to detect BUB3 in ELISA, WB applications, with IgG isotype.
Reported reactive against Human, Mouse, Rat. Immunogen derived from Human. Cross-reactivity with other species should not be assumed without documented data or empirical testing.
Reported for ELISA, WB. Each format requires independent dilution optimization and appropriate controls. Unlisted applications require empirical testing.
This non-conjugated IgG antibody requires a compatible secondary. Select anti-rabbit IgG secondary conjugated to your preferred reporter (HRP, AP, fluorophore, or biotin), matched to IgG.
(1) Isotype control — IgG from Rabbit at matching concentration; (2) Positive control — known BUB3-expressing cell/tissue; (3) Negative control — knockdown/knockout sample for specificity; (4) Dilution linearity — verify proportional signal decrease in your matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
