| Field | Specification |
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| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human Phospho-c-Jun (S73)+JunD (S100) was used as the immunogen for the Phospho-c-Jun/JunD (Ser73/Ser100) antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Phospho-c-Jun/JunD (Ser73/Ser100) Antibody is a anti-c-Jun/JunD (phospho-Ser73, Ser100) Rabbit antibody Recombinant Rabbit Monoclonal clone 30J61 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP) with listed reactivity in Human, Mouse.
Key elements and design rationale
- Target: c-Jun/JunD (phospho site: Ser73, Ser100)
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30J61, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, IP
Biological background
Phospho-c-Jun/JunD (Ser73/Ser100) antibody is widely applied in cancer biology, immunology, and stress signaling research. Detection of phosphorylated AP-1 proteins provides insight into MAPK signaling cascades that drive transcriptional reprogramming. Abnormal AP-1 activation contributes to oncogenesis, inflammation, and neurodegeneration. By detecting phosphorylated c-Jun and JunD, researchers can examine how stress-activated kinases influence gene regulation and disease progression.
Western blot assays reveal phosphorylated isoforms distinct from non-phosphorylated proteins. Immunohistochemistry maps AP-1 activation across tissues, while immunofluorescence highlights nuclear localization of phosphorylated c-Jun and JunD. These techniques allow high-resolution tracking of transcription factor activation in diverse biological contexts.
Phosphorylation of c-Jun and JunD integrates signals from growth factors, UV radiation, oxidative stress, and cytokines. Sustained phosphorylation is often linked to tumorigenesis and resistance to therapy, while transient activation supports adaptive responses. By applying Phospho-c-Jun/JunD (Ser73/Ser100) antibody, scientists can evaluate pathway dynamics and investigate therapeutic strategies targeting JNK-AP1 signaling in cancer and inflammation.
Beyond oncology, phosphorylated AP-1 proteins play roles in embryonic development, neuronal plasticity, and immune activation. Their regulation of genes involved in apoptosis, differentiation, and repair underscores their biological importance. The phospho-specific antibody therefore provides a versatile reagent for dissecting cellular responses to external and internal cues.
Phospho-c-Jun/JunD (Ser73/Ser100) antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Quantifying post-translational regulation (including phosphorylation) alongside total protein levels.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Phospho-site readouts are condition-dependent and are often compared to total target levels when available.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
