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| Immunogen | A synthesized peptide derived from human Phospho-CaMKII alpha (T286) was used as the immunogen for the Phospho-CaMKII alpha (T286) antibody. Due to the conserved nature of the epitope, the antibody will likely also detect Phospho-CaMKII beta (T287). |
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Overview
Phospho-CaMKII alpha (T286) Antibody / CAMK2A is a anti-CAMK2A (phospho-Thr286) Rabbit antibody Recombinant Rabbit Monoclonal clone 31C26 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Mouse, Rat.
Key elements and design rationale
- Target: CAMK2A (phospho site: Thr286)
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31C26, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC
Biological background
Phospho-CaMKII alpha (T286) antibody is widely applied in neuroscience, synaptic biology, and signaling research. Detecting phosphorylation at this site provides a direct measure of CaMKII activation, linking cellular activity to memory formation. In neurons, Thr286 phosphorylation prolongs kinase activity beyond transient calcium spikes, enabling sustained synaptic strengthening. By targeting this phosphorylation event, researchers can explore how activity-dependent signaling translates into lasting changes in connectivity.
Applications for Phospho-CaMKII alpha (T286) antibody include western blotting, immunohistochemistry, immunofluorescence, and ELISA. Western blot assays detect phosphorylated CaMKII alpha isoforms in brain lysates, immunohistochemistry maps activation in hippocampus and cortex, and immunofluorescence reveals localization at dendritic spines. These methods provide detailed views of synaptic signaling in vitro and in vivo.
Phosphorylation at Thr286 has been extensively studied in relation to long-term potentiation, a cellular mechanism of memory storage. Abnormal regulation of CaMKII phosphorylation contributes to neurological disorders, including Alzheimer disease, schizophrenia, and epilepsy. By applying Phospho-CaMKII alpha (T286) antibody, scientists can investigate disease-related disruptions in synaptic plasticity.
Beyond neuroscience, CaMKII alpha also participates in cardiac signaling and metabolism. Phosphorylation at Thr286 has been detected in cardiac stress responses and metabolic regulation. The antibody therefore supports research into CaMKII biology across systems.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Quantifying post-translational regulation (including phosphorylation) alongside total protein levels.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Phospho-site readouts are condition-dependent and are often compared to total target levels when available.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
