| Field | Specification |
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| Host | |
| Immunogen | A synthesized peptide derived from human Phospho-Caveolin-1 (Y14) was used as the immunogen for the Phospho-Caveolin 1 (Tyr14) antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
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| Target | |
| UniProt # |
Overview
Phospho-Caveolin 1 (Tyr14) Antibody / CAV1 is a anti-CAV1 (phospho-Tyr14) Rabbit antibody Recombinant Rabbit Monoclonal clone 32C49 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.
Key elements and design rationale
- Target: CAV1 (phospho site: Tyr14)
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32C49, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
Caveolin 1 acts as a scaffolding protein that organizes signaling complexes at the plasma membrane. Research using Phospho-Caveolin 1 (Tyr14) antibody has demonstrated that phosphorylation at tyrosine 14 is mediated primarily by Src family kinases in response to growth factors, mechanical stress, or cellular adhesion events. This modification induces conformational changes that modulate protein interactions and influence downstream signaling. By altering binding partners, phosphorylation at Tyr14 affects processes such as focal adhesion turnover, cell migration, and proliferation.
Studies with Phospho-Caveolin 1 (Tyr14) antibody have shown that this modification regulates integrin signaling and cytoskeletal dynamics. Phosphorylated Caveolin 1 promotes disassembly of focal adhesions, allowing cells to migrate during wound healing, angiogenesis, and cancer metastasis. It also participates in mechanotransduction, enabling cells to sense and respond to changes in extracellular matrix stiffness or fluid shear stress. These findings highlight the versatility of Caveolin 1 phosphorylation as a regulatory switch in cell biology.
Dysregulation of Caveolin 1 phosphorylation has been linked to multiple diseases. Research using Phospho-Caveolin 1 (Tyr14) antibody has revealed altered phosphorylation in cancer, where excessive modification promotes tumor cell invasion and metastasis. In cardiovascular disease, abnormal Caveolin 1 phosphorylation disrupts endothelial function and contributes to vascular pathology. In pulmonary fibrosis, changes in Caveolin 1 phosphorylation status affect fibroblast activation and extracellular matrix remodeling. These diverse roles emphasize its broad clinical relevance.
Phospho-Caveolin 1 (Tyr14) antibody is widely used in western blotting, immunohistochemistry, and immunofluorescence. Western blotting distinguishes phosphorylated Caveolin 1 from its unmodified form, immunohistochemistry identifies activated signaling domains in tissues, and immunofluorescence visualizes phosphorylated Caveolin 1 within caveolae at the plasma membrane. These applications make the antibody indispensable for cell signaling and membrane biology research.
By providing validated Phospho-Caveolin 1 (Tyr14) antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Quantifying post-translational regulation (including phosphorylation) alongside total protein levels.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Phospho-site readouts are condition-dependent and are often compared to total target levels when available.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
