| Field | Specification |
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| Immunogen | A synthesized peptide derived from human Phospho-CDK2(T160)+CDK1(T161) was used as the immunogen for the Phospho-CDK2/CDK1 (Thr160/Thr161) antibody. |
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| Target | |
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Overview
Phospho-CDK2/CDK1 (Thr160/Thr161) Antibody is a anti-CDK1 (phospho-Thr160, Thr161) Rabbit antibody Recombinant Rabbit Monoclonal clone 31C95 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human, Mouse. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: CDK1 (phospho site: Thr160, Thr161)
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31C95, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC
Biological background
Phospho-CDK2/CDK1 (Thr160/Thr161) antibody is widely applied in cancer biology, cell cycle research, and molecular pharmacology. Detection of phosphorylation at these residues provides a direct measure of CDK activation status. In proliferating cells, CDK2 in complex with cyclin E or cyclin A promotes DNA replication, while CDK1 in complex with cyclin B drives mitotic entry. By monitoring phosphorylation at Thr160 and Thr161, researchers can assess how CDK activation is controlled during the cell cycle and disrupted in disease.
In western blot assays, Phospho-CDK2/CDK1 (Thr160/Thr161) antibody detects phosphorylated isoforms distinct from inactive CDK2 and CDK1. Immunohistochemistry highlights nuclear staining in actively cycling cells within tissues, while immunofluorescence reveals subcellular localization of active kinase complexes at replication foci and mitotic structures. These methods provide powerful tools for visualizing CDK activity in situ.
CDK2 and CDK1 phosphorylation is regulated by cyclin binding and CDK activating kinase (CAK). In addition to activating phosphorylation, inhibitory phosphorylation at other residues fine tunes kinase activity, ensuring proper timing of cell cycle transitions. Aberrant phosphorylation results in unscheduled proliferation, genomic instability, and oncogenesis. By applying Phospho-CDK2/CDK1 (Thr160/Thr161) antibody, scientists can study how signaling pathways converge on CDK regulation and explore therapeutic strategies that target cell cycle kinases.
In oncology, dysregulated CDK2 and CDK1 activity is a hallmark of many tumors. Overexpression of cyclins, loss of CDK inhibitors such as p21 or p27, and altered phosphorylation lead to unchecked proliferation. Small molecule inhibitors of CDKs are under clinical development, and monitoring phosphorylation status provides a biomarker for therapeutic efficacy. This antibody therefore supports translational research linking kinase signaling to cancer therapy.
Beyond cancer, CDK2 and CDK1 phosphorylation contributes to developmental biology, stem cell regulation, and tissue regeneration. Phosphorylation at Thr160 and Thr161 ensures fidelity of DNA replication, chromosome segregation, and genome stability. Dysregulation is implicated in infertility, developmental syndromes, and age related decline. The phospho-specific antibody provides a means to evaluate CDK function across diverse biological systems.
Phosphorylation dynamics also link CDKs to DNA damage response and stress signaling. Upon genotoxic stress, inhibitory pathways suppress CDK activity to allow repair before replication or division. Conversely, inappropriate activation leads to replication stress and apoptosis. By using Phospho-CDK2/CDK1 (Thr160/Thr161) antibody, researchers can examine how stress signals intersect with cell cycle progression and contribute to disease mechanisms.
Phospho-CDK2/CDK1 (Thr160/Thr161) antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Quantifying post-translational regulation (including phosphorylation) alongside total protein levels.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Phospho-site readouts are condition-dependent and are often compared to total target levels when available.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
