| Field | Specification |
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| Host | |
| Immunogen | A synthesized peptide derived from human Phospho-CrkL (Y207) was used as the immunogen for the Phospho-CrkL (Tyr207) antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
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| Target | |
| UniProt # |
Overview
Phospho-CrkL (Tyr207) Antibody / Crk like protein is a anti-CrkL (phospho-Tyr207) Rabbit antibody Recombinant Rabbit Monoclonal clone 32C08 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.
Key elements and design rationale
- Target: CrkL (phospho site: Tyr207)
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32C08, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
Crk like phosphorylation at tyrosine 207 is mediated by kinases such as ABL and has been shown to modulate protein conformation and adaptor activity. Research using Phospho-CrkL (Tyr207) antibody has demonstrated that phosphorylation reduces binding to certain partners while promoting interactions with negative regulators, thus acting as a feedback mechanism to control signaling intensity. This fine tuning ensures that pathways such as Ras, Rac, and PI3K are activated appropriately in response to growth factor stimulation or stress signals.
Aberrant phosphorylation of Crk like at tyrosine 207 has been strongly associated with leukemia, particularly chronic myeloid leukemia, where BCR ABL fusion kinase constitutively phosphorylates this residue. Phospho-CrkL (Tyr207) antibody has therefore become a surrogate marker for BCR ABL activity in both research and clinical contexts. Monitoring Crk like phosphorylation provides a sensitive measure of kinase inhibition in patients treated with tyrosine kinase inhibitors, making it a valuable biomarker for therapeutic response. Beyond leukemia, altered phosphorylation has been observed in solid tumors, linking Crk like to diverse oncogenic pathways.
Phospho-CrkL (Tyr207) antibody is used in western blotting, immunoprecipitation, and flow cytometry. Western blotting provides detection of phosphorylated protein as an indicator of kinase activity. Immunoprecipitation with this antibody isolates Crk like protein complexes, allowing characterization of binding partners under different phosphorylation states. Flow cytometry applications extend its use to patient samples, where phosphorylation levels can be measured at the single cell level. These diverse applications demonstrate the versatility of Phospho-CrkL (Tyr207) antibody in both laboratory research and translational medicine.
By providing Phospho-CrkL (Tyr207) antibody,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Quantifying post-translational regulation (including phosphorylation) alongside total protein levels.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Phospho-site readouts are condition-dependent and are often compared to total target levels when available.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
