| Field | Specification |
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| Immunogen | A synthesized peptide derived from human Phospho-eIF4EBP1/2/3 (T46+T46+T32) was used as the immunogen for the Phospho-eIF4EBP1 (Thr46) antibody. |
| Isotype | |
| Product Type | |
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| Target | |
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Overview
Phospho-eIF4EBP1 (Thr46) Antibody / Eukaryotic translation initiation factor 4E binding protein 1 is a anti-EIF4EBP1 (phospho-Thr46) Rabbit antibody Recombinant Rabbit Monoclonal clone 32E52 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human, Mouse.
Key elements and design rationale
- Target: EIF4EBP1 (phospho site: Thr46)
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32E52, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC
Biological background
Phospho-eIF4EBP1 (Thr46) antibody is widely applied in studies of mTOR signaling, translation control, and cancer biology. The mTOR pathway is a central regulator of cell growth, proliferation, and metabolism, and EIF4EBP1 phosphorylation is one of its key downstream events. By specifically detecting EIF4EBP1 phosphorylated at Thr46, this antibody provides a reliable marker of pathway activation and translational regulation.
The antibody is suitable for western blotting, immunohistochemistry, and immunofluorescence. In western blot assays, Phospho-eIF4EBP1 (Thr46) antibody identifies phosphorylated protein bands distinct from non phosphorylated forms, allowing quantification of activation states. Immunohistochemistry provides tissue level mapping of phosphorylation patterns, while immunofluorescence reveals subcellular localization of activated EIF4EBP1. These applications make the antibody valuable for monitoring mTOR activity across experimental models.
EIF4EBP1 phosphorylation at Thr46 is conserved among family members, and antibodies raised against this site may also detect EIF4EBP2 at Thr46 and EIF4EBP3 at Thr32. This cross reactivity can be useful in capturing broader regulation of translation initiation but should be considered in experimental design. By applying Phospho-eIF4EBP1 (Thr46) antibody, researchers can evaluate translational control across multiple EIF4EBP proteins.
Dysregulation of EIF4EBP1 phosphorylation is common in cancers, where hyperactivation of mTOR signaling drives uncontrolled growth and survival. Detection of phospho EIF4EBP1 is frequently used as a biomarker of therapeutic response to mTOR inhibitors. Beyond oncology, mTOR EIF4EBP1 signaling regulates metabolism, neuronal plasticity, and immune responses, expanding the relevance of this antibody to diverse research fields.
Phospho-eIF4EBP1 (Thr46) antibody provided by
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Quantifying post-translational regulation (including phosphorylation) alongside total protein levels.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Phospho-site readouts are condition-dependent and are often compared to total target levels when available.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
