| Field | Specification |
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| Immunogen | A synthesized peptide derived from human Phospho-FAK (Y576 + Y577) was used as the immunogen for the Phospho-FAK (Tyr576/Tyr577) antibody. |
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Overview
Phospho-FAK (Tyr576/Tyr577) Antibody / Focal Adhesion Kinase 1 is a anti-FAK (phospho-Tyr576, Tyr577) Rabbit antibody Recombinant Rabbit Monoclonal clone 32P46 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse.
Key elements and design rationale
- Target: FAK (phospho site: Tyr576, Tyr577)
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32P46, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
Phospho-FAK (Tyr576/Tyr577) antibody is widely used in cancer biology, cell adhesion studies, and mechanotransduction research. Active FAK drives tumor progression, metastasis, and angiogenesis by promoting migration and survival of cancer cells. Detection of phosphorylation at Tyr576/577 provides a direct measure of FAK activation and signaling output. By using this antibody, researchers can evaluate FAK driven pathways in oncogenesis, wound healing, and fibrosis.
Western blot assays distinguish phosphorylated FAK from unmodified protein, while immunohistochemistry maps activation in tissues undergoing remodeling or tumor progression. Immunofluorescence highlights localization of phospho-FAK at focal adhesions, providing spatial insight into adhesion-dependent signaling events. These approaches allow comprehensive analysis of FAK activation in different systems.
Phosphorylation at Tyr576 and Tyr577 is essential for full kinase activation. It promotes conformational changes that enhance substrate binding and signaling complex assembly. Dysregulated FAK phosphorylation contributes to cancer cell invasion and therapy resistance. By applying Phospho-FAK (Tyr576/Tyr577) antibody, scientists can study therapeutic strategies that target adhesion-dependent kinase activity and its role in disease.
FAK signaling also participates in cardiovascular and developmental biology, where it regulates vascular remodeling, stem cell adhesion, and tissue morphogenesis. Its integration of mechanical and biochemical signals makes it a central regulator of cell behavior. The phospho-specific antibody therefore serves as a powerful tool for examining mechanosensitive signaling networks.
Phospho-FAK (Tyr576/Tyr577) antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Quantifying post-translational regulation (including phosphorylation) alongside total protein levels.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Phospho-site readouts are condition-dependent and are often compared to total target levels when available.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
