| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide specific to the region of human MDM2 protein surrounding phosphorylated serine 166 was used as the immunogen for the Phospho-MDM2 antibody (pS166). |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Phospho-MDM2 Antibody (pS166) is an antibody targeting PHOSPHO-MDM2, raised in Rabbit for protein detection and localization studies where these specifications are required.
Key elements and design rationale
- Target: PHOSPHO-MDM2.
- Antibody identity: Recombinant Rabbit Monoclonal; Clone 29M35; Rabbit IgG.
- Conjugate/label: Unconjugated (affects detection chemistry and multiplex compatibility).
- Format: Purified.
- Species reactivity: Human, Mouse, Rat.
- Listed applications: WB, IF, IHC-P (refer to on-page specifications for application-specific guidance).
Biological background
Phosphorylated MDM2 is a post-translationally modified form of the MDM2 protein, an E3 ubiquitin ligase that regulates the stability and activity of the tumor suppressor p53. Phosphorylation of MDM2 at specific residues modulates its localization, interaction with binding partners, and overall activity in the p53 pathway. Researchers often use a Phospho-MDM2 antibody to study these regulatory mechanisms.
Cellular stress signals such as DNA damage or growth factor stimulation can trigger phosphorylation of MDM2, altering its ability to degrade p53 and impacting cell cycle progression, DNA repair, and apoptosis. Employing a Phospho-MDM2 antibody enables detection of these phosphorylation-dependent changes and provides insights into signaling cascades that control cell fate.
NSJ Bioreagents offers a high-quality Phospho-MDM2 antibody for applications including western blot, immunoprecipitation, and immunofluorescence. Selecting the right Phospho-MDM2 antibody ensures reliable detection of this critical regulatory modification in cancer biology, cell cycle control, and stress response studies.
Research relevance and current trends
- Comparative expression profiling across cell types, tissues, or perturbations (e.g., drug treatment, genetic editing, or differentiation).
- Subcellular localization and trafficking studies, including co-localization with pathway markers in microscopy-based assays.
- Integration of protein-level measurements with transcriptomics or proteomics to relate abundance to regulation and phenotype.
Common research applications
- Western blotting: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
- Immunofluorescence: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
- Immunohistochemistry: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
Interpretation should account for antibody-dependent factors such as epitope accessibility, isoforms, and sample preparation differences across workflows.
Notes for experimental interpretation
- Isoforms and PTMs: many targets have multiple isoforms and post-translational modifications that can shift apparent signal or localization; interpret bands/signals accordingly.
- Epitope context: binding can depend on protein conformation and sample processing; region information in the title/immunogen can help anticipate what may be detected.
- Species differences: predicted or validated reactivity may vary by ortholog sequence and sample context; confirm in your model system.
- Control concepts: include negative controls (no-primary/isotype), and where possible genetic controls (KO/KD) or independent antibodies to strengthen conclusions.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
