| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human Phospho-Nucleophosmin (S125) was used as the immunogen for the Phospho-Nucleophosmin (Ser125) antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Phospho-Nucleophosmin (Ser125) Antibody / NPM1 is a anti-NPM1 (phospho-Ser125) Rabbit antibody Recombinant Rabbit Monoclonal clone 32N71 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: NPM1 (phospho site: Ser125)
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32N71, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC
Biological background
Nucleophosmin is abundant in the nucleolus, where it functions in ribosomal RNA processing and assembly of ribosomal subunits. Phosphorylation at serine 125 occurs during mitosis, and research with Phospho-Nucleophosmin (Ser125) antibody has revealed that this modification promotes centrosome separation and spindle formation. By regulating Nucleophosmin function, phosphorylation ensures faithful chromosome segregation and prevents aneuploidy. Disruption of this modification impairs mitotic progression and contributes to genomic instability, highlighting its importance in cell division control.
NPM1 is also a key player in hematological malignancies. Mutations in NPM1, particularly frame-shift mutations in exon 12, are among the most frequent genetic alterations in acute myeloid leukemia. These mutations cause aberrant cytoplasmic localization of Nucleophosmin, altering its regulatory functions. Research using Phospho-Nucleophosmin (Ser125) antibody has shown that phosphorylation state influences localization and protein interactions, potentially modulating leukemogenesis. Furthermore, phosphorylation may interact with mutational effects, making it an area of interest in understanding leukemia biology and therapy.
Beyond cancer, Nucleophosmin phosphorylation contributes to stress responses, DNA repair, and apoptosis regulation. Phospho-Nucleophosmin (Ser125) antibody has been used to demonstrate that cellular stress induces phosphorylation at this site, altering interactions with ARF and p53. These findings suggest a broader role for Nucleophosmin phosphorylation in tumor suppression and cell fate decisions. Since Nucleophosmin participates in chromatin remodeling, this modification may also influence transcription and epigenetic regulation.
Phospho-Nucleophosmin (Ser125) antibody is widely applied in western blotting, immunofluorescence, and immunohistochemistry. Western blotting distinguishes phosphorylated from unmodified forms, while immunofluorescence visualizes dynamic localization changes during the cell cycle. Immunohistochemistry allows detection of phosphorylation patterns in tumor tissue, linking biochemical modification to disease phenotype. These experimental approaches provide insights into the regulation of Nucleophosmin in health and disease.
By supplying validated Phospho-Nucleophosmin (Ser125) antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Quantifying post-translational regulation (including phosphorylation) alongside total protein levels.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Phospho-site readouts are condition-dependent and are often compared to total target levels when available.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
