| Field | Specification |
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| Host | |
| Immunogen | A synthesized peptide derived from human Phospho-p38 (T180) was used as the immunogen for the Phospho-p38 (Thr180) antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
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| Target | |
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Overview
Phospho-p38 (Thr180) Antibody / MAPK14 is a anti-P38 (phospho-Thr180) Rabbit antibody Recombinant Rabbit Monoclonal clone 31M76 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human.
Key elements and design rationale
- Target: P38 (phospho site: Thr180)
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31M76, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC
Biological background
p38 mitogen activated protein kinase regulates a wide range of biological processes, including transcription, cell cycle arrest, apoptosis, and cytokine production. Following phosphorylation at threonine 180, p38 phosphorylates substrates such as ATF2, MAPKAPK2, and HSP27, linking stress signals to gene expression and cytoskeletal remodeling. Studies with Phospho-p38 (Thr180) antibody have shown that pathway activation is transient and tightly controlled, ensuring that cellular stress responses are adaptive rather than destructive. Dysregulation of this phosphorylation event contributes to inflammatory disease, cancer, and neurodegeneration.
In immune biology, p38 activation promotes the production of cytokines such as TNF alpha, IL6, and IL1 beta. Research using Phospho-p38 (Thr180) antibody has demonstrated that inhibition of p38 phosphorylation reduces inflammatory cytokine output, supporting therapeutic strategies targeting this pathway. In oncology, altered p38 signaling influences tumor cell apoptosis, angiogenesis, and metastasis, making it a subject of active drug development. In neurons, aberrant phosphorylation contributes to synaptic dysfunction and degenerative disease.
Phospho-p38 (Thr180) antibody is widely used in western blotting, immunohistochemistry, and immunofluorescence. Western blotting reveals activation status across experimental conditions, while immunohistochemistry highlights tissue specific phosphorylation patterns. Immunofluorescence demonstrates subcellular distribution of active kinase in stressed cells. These applications provide critical insights into stress signaling, immune regulation, and pathological responses.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Quantifying post-translational regulation (including phosphorylation) alongside total protein levels.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Phospho-site readouts are condition-dependent and are often compared to total target levels when available.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
