| Field | Specification |
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| Host | |
| Immunogen | A synthesized peptide derived from human Phospho-PBK/TOPK (Thr9) was used as the immunogen for the Phospho-PBK (Thr9) Antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
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| UniProt # |
Overview
Phospho-PBK (Thr9) Antibody / PDZ binding kinase is a anti-PBK (phospho-Thr9) Rabbit antibody Recombinant Rabbit Monoclonal clone 30P33 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human.
Key elements and design rationale
- Target: PBK (phospho site: Thr9)
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30P33, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC
Biological background
Phospho-PBK (Thr9) antibody is widely applied in oncology, immunology, and cell cycle research. PBK activation supports G2/M transition by phosphorylating downstream substrates involved in spindle formation and chromosome segregation. It also participates in immune signaling pathways in T cells and NK cells. By detecting phosphorylation at Thr9, researchers can monitor PBK activation and its role in proliferation and immunity.
Applications for Phospho-PBK (Thr9) antibody include western blotting, immunohistochemistry, and immunofluorescence. Western blot assays detect phosphorylated PBK isoforms, immunohistochemistry maps expression in tumors, and immunofluorescence reveals subcellular localization at centrosomes during mitosis. These complementary approaches provide detailed evaluation of PBK biology.
Dysregulated PBK activity is implicated in multiple cancers, including lung, breast, and hematologic malignancies. Elevated Thr9 phosphorylation correlates with tumor grade and poor prognosis, reflecting hyperactive proliferation. In immune biology, PBK phosphorylation regulates lymphocyte activation, linking cell cycle control to host defense. By applying Phospho-PBK (Thr9) antibody, scientists can study these dual roles in cancer and immunity.
Therapeutic research has targeted PBK as a potential kinase for inhibition. Small molecule inhibitors reduce proliferation and sensitize tumor cells to chemotherapy. Monitoring Thr9 phosphorylation provides a pharmacodynamic readout of drug activity. Phospho-PBK (Thr9) antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Quantifying post-translational regulation (including phosphorylation) alongside total protein levels.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Phospho-site readouts are condition-dependent and are often compared to total target levels when available.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
