| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | The amino acids surrounding phosphorylated threonine 17 from the human protein were used as the immunogen for the Phospho-PBL antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Phospho-PBL Antibody / Phospholamban (Phospho-T17) is an antibody targeting PHOSPHO-PBL, raised in Rabbit for protein detection and localization studies where these specifications are required.
Key elements and design rationale
- Target: PHOSPHO-PBL.
- Antibody identity: Polyclonal (rabbit origin); Rabbit Ig.
- Conjugate/label: Unconjugated (affects detection chemistry and multiplex compatibility).
- Format: Antigen affinity purified.
- Species reactivity: Human.
- Listed applications: WB, DB (refer to on-page specifications for application-specific guidance).
Biological background
Phospholamban is a small protein found in the sarcoplasmic reticulum of cardiac muscle cells, where it plays a crucial role in regulating the calcium ion concentration and ultimately the contraction of the heart muscle. Phosphorylated phospholamban is a form of the protein that has undergone a chemical modification in which phosphate groups are added to specific amino acid residues. This modification has been found to alter the interaction of phospholamban with other proteins and enzymes in the cell, leading to changes in calcium handling and cardiac contractility. Studies have demonstrated that phosphorylated phospholamban can enhance the function of the sarcoplasmic reticulum calcium pump, which is responsible for pumping calcium ions out of the cell after each contraction. This increased activity results in improved relaxation of the heart muscle and overall cardiac function. Furthermore, research has shown that abnormalities in phosphorylated phospholamban levels are associated with various cardiac conditions, including heart failure and arrhythmias.
Research relevance and current trends
- Comparative expression profiling across cell types, tissues, or perturbations (e.g., drug treatment, genetic editing, or differentiation).
- Subcellular localization and trafficking studies, including co-localization with pathway markers in microscopy-based assays.
- Integration of protein-level measurements with transcriptomics or proteomics to relate abundance to regulation and phenotype.
Common research applications
- Western blotting: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
- DB: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
Interpretation should account for antibody-dependent factors such as epitope accessibility, isoforms, and sample preparation differences across workflows.
Notes for experimental interpretation
- Isoforms and PTMs: many targets have multiple isoforms and post-translational modifications that can shift apparent signal or localization; interpret bands/signals accordingly.
- Epitope context: binding can depend on protein conformation and sample processing; region information in the title/immunogen can help anticipate what may be detected.
- Species differences: predicted or validated reactivity may vary by ortholog sequence and sample context; confirm in your model system.
- Control concepts: include negative controls (no-primary/isotype), and where possible genetic controls (KO/KD) or independent antibodies to strengthen conclusions.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
