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| Immunogen | A synthesized peptide derived from human Phospho-PKA C (Thr197) was used as the immunogen for the Phospho-PKA C (Thr197) antibody. |
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Overview
Phospho-PKA C (Thr197) Antibody / PRKACA/PRKACB/PRKACG is a anti-PKA (phospho-Thr197) Rabbit antibody Recombinant Rabbit Monoclonal clone 32P41 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: PKA (phospho site: Thr197)
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32P41, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC
Biological background
Protein kinase A catalytic subunit is activated when cAMP binds regulatory subunits, releasing the catalytic subunits into the cytoplasm. Research using Phospho-PKA C (Thr197) antibody has demonstrated that phosphorylation at threonine 197 occurs within the activation loop and is necessary for stabilizing the active conformation. This phosphorylation is typically mediated by phosphoinositide-dependent kinase 1 and ensures robust catalytic activity toward downstream substrates. Without this modification, kinase activity is impaired, highlighting its essential role in PKA signaling.
Studies with Phospho-PKA C (Thr197) antibody have revealed that phosphorylation of the catalytic subunit regulates diverse cellular processes. These include glycogen metabolism, lipolysis, ion channel regulation, and transcriptional activation. PKA phosphorylates transcription factors such as CREB, influencing gene expression programs in response to hormonal and metabolic signals. By monitoring phosphorylation at Thr197, researchers gain insight into dynamic activation of cAMP-dependent signaling.
Dysregulation of PKA phosphorylation has been linked to disease. Research using Phospho-PKA C (Thr197) antibody has shown that abnormal activity contributes to endocrine disorders, cardiovascular disease, and cancer. Mutations in PRKACA are associated with adrenal hyperplasia and Cushing's syndrome, conditions characterized by excessive PKA signaling. In oncology, altered phosphorylation of PKA subunits affects cell proliferation and survival, supporting tumor growth. These findings emphasize the importance of Thr197 phosphorylation in physiology and pathology.
Phospho-PKA C (Thr197) antibody is widely applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting distinguishes active phosphorylated subunits, immunohistochemistry highlights activated kinase in tissues, and immunofluorescence demonstrates localization of phosphorylated subunits in response to stimuli. These applications make Phospho-PKA C (Thr197) antibody an indispensable tool in signaling research.
By supplying validated Phospho-PKA C (Thr197) antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Quantifying post-translational regulation (including phosphorylation) alongside total protein levels.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Phospho-site readouts are condition-dependent and are often compared to total target levels when available.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
