| Field | Specification |
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| Immunogen | A synthesized peptide derived from human Phospho-PKC delta (T507) was used as the immunogen for the Phospho-PKC delta (Thr507) antibody. |
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Overview
Phospho-PKC delta (Thr507) Antibody / Protein kinase C delta is a anti-PKC (phospho-Thr507) Rabbit antibody Recombinant Rabbit Monoclonal clone 32P74 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.
Key elements and design rationale
- Target: PKC (phospho site: Thr507)
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32P74, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
Phospho-PKC delta Thr507 antibody is widely used to study kinase signaling and phosphorylation dependent regulation. By specifically detecting PKC delta in its activated phosphorylated form, the antibody allows researchers to distinguish between inactive and active signaling states. This is particularly important in studies of cancer, cardiovascular disease, and inflammation, where PKC delta activity is tightly regulated and often dysregulated.
The antibody is suitable for western blotting, immunohistochemistry, and immunofluorescence. In western blot assays, Phospho PKC delta Thr507 antibody detects phosphorylated isoforms of the kinase, enabling quantification of activation under different treatments. In immunohistochemistry, it reveals phosphorylation dependent localization within tissues, highlighting regions of signaling activation. Immunofluorescence experiments allow visualization of PKC delta phosphorylation at the single cell level, providing insight into dynamic regulatory events.
PKC delta has been implicated in pro apoptotic signaling pathways, where activation at Thr507 contributes to programmed cell death. Conversely, in certain cancer contexts, PKC delta activity promotes survival and proliferation. Monitoring Thr507 phosphorylation using phospho specific antibodies provides mechanistic understanding of how PKC delta signaling outcomes vary depending on cellular context. In cardiovascular biology, PKC delta contributes to ischemia reperfusion injury and vascular remodeling, making its phosphorylation status a useful biomarker of stress responses.
Phospho-PKC delta Thr507 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Quantifying post-translational regulation (including phosphorylation) alongside total protein levels.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Phospho-site readouts are condition-dependent and are often compared to total target levels when available.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
